Overview

  • Product name
  • Description
    Goat polyclonal to MYO1B
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Chinese hamster
  • Immunogen

    Synthetic peptide:

    C-RTVEAKQEKVSTT

    (with a Cysteine residue linker), corresponding to internal sequence amino acids 327-339 of Human MYO1B (O43795; NP_001123630.1; NP_036355.2).

  • Positive control
    • NIH3T3 lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab126865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 132 kDa.

Target

Images

  • ab126865 (5µg/ml) shows staining of MYO1B in paraffin embedded Human Cerebral Cortex following steamed antigen retrieval with citrate buffer pH 6 and AP-staining. This shows cytoplasm staining in the neuronal bodies.

  • Anti-MYO1B antibody (ab126865) at 1 µg/ml + NIH3T3 lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 132 kDa


    Exposure time : 1 hour

References

ab126865 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (mouse embryo E1.5 and E2.5)
Specification
mouse embryo E1.5 and E2.5
Permeabilization
Yes - Triton X100 0.1%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up