Anti-MYO1D antibody (ab70204)
Key features and details
- Mouse polyclonal to MYO1D
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MYO1D antibody -
Description
Mouse polyclonal to MYO1D -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human MYO1D. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- MYO1D transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
Predicted molecular weight for isoform 2: 50 kDa. |
Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). Predicted molecular weight for isoform 2: 50 kDa. |
Target
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Relevance
Myosins are actin based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. -
Database links
- Entrez Gene: 4642 Human
- Omim: 606539 Human
- SwissProt: O94832 Human
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Alternative names
- KIAA0727 antibody
- Myosin I gamma antibody
- Myosin Id antibody
- MYR4 antibody
Images
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All lanes : Anti-MYO1D antibody (ab70204) at 1/500 dilution
Lane 1 : MYO1D transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab70204 has been referenced in 1 publication.
- Saydmohammed M et al. Vertebrate myosin 1d regulates left-right organizer morphogenesis and laterality. Nat Commun 9:3381 (2018). PubMed: 30139971