Overview

  • Product name
  • Description
    Rabbit polyclonal to MYO5A
  • Specificity
    ab110468 detects endogenous levels of total MYO5A protein.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from the C terminal region of Human MYO5A (UniProt ID: Q9Y4I1).

  • Positive control
    • Extracts from Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab110468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 215 kDa.

Target

  • Function
    Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. Also mediates the transport of vesicles to the plasma membrane. May also be required for some polarization process involved in dendrite formation.
  • Tissue specificity
    Detected in melanocytes.
  • Involvement in disease
    Griscelli syndrome 1
    Griscelli syndrome 3
    Elejalde syndrome
  • Sequence similarities
    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
    Contains 1 dilute domain.
    Contains 6 IQ domains.
    Contains 1 myosin motor domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • non-muscle antibody
    • Dilute myosin heavy chain antibody
    • GS1 antibody
    • MYH12 antibody
    • MYO5 antibody
    • Myo5a antibody
    • MYO5A_HUMAN antibody
    • Myosin heavy chain 12 antibody
    • Myosin heavy polypeptide kinase antibody
    • Myosin V antibody
    • Myosin VA (heavy polypeptide 12 myoxin) antibody
    • Myosin VA (heavy polypeptide 12 myoxin) antibody
    • Myosin-12 antibody
    • Myoxin antibody
    • MYR12 antibody
    • Unconventional myosin-Va antibody
    see all

Images

  • All lanes : Anti-MYO5A antibody (ab110468) at 1/500 dilution

    Lane 1 : Extracts from Hela cells
    Lane 2 : Extracts from Hela cells, treated with the synthesized peptide

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 215 kDa

References

ab110468 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
10 µg
Specification
Brain
Blocking step
BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ansley Kunnath

Verified customer

Submitted Jul 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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