Overview

  • Product name
  • Description
    Rabbit polyclonal to MYO5B
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to C-terminal residues of human MYO5B

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 214 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Function
    May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.
  • Involvement in disease
    Diarrhea 2, with microvillus atrophy
  • Sequence similarities
    Contains 1 dilute domain.
    Contains 6 IQ domains.
    Contains 1 myosin head-like domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1119 antibody
    • Myo5b antibody
    • MYO5B_HUMAN antibody
    • Myosin Vb antibody
    • Unconventional myosin-Vb antibody
    see all

References for Anti-MYO5B antibody (ab58765)

This product has been referenced in:
  • Gao N & Kaestner KH Cdx2 regulates endo-lysosomal function and epithelial cell polarity. Genes Dev 24:1295-305 (2010). WB ; Mouse . Read more (PubMed: 20551175) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Rat Cell lysate - whole cell (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 17 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
500000 cells
Gel Running Conditions
Reduced Denaturing (4-12% bis-tris NUPAGE invitrogen)
Sample
Human Cell lysate - whole cell (bladder cancer)
Specification
bladder cancer
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Thuy Trang

Verified customer

Submitted Aug 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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