Overview

  • Product nameAnti-Myocardin antibody
    See all Myocardin primary antibodies
  • Description
    Rabbit polyclonal to Myocardin
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal domain of Human Myocardin.

Properties

Applications

Our Abpromise guarantee covers the use of ab106346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 102 kDa.
IHC-Fr 1/50 - 1/500.

Target

  • FunctionTranscriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
  • Tissue specificityExpressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
  • Sequence similaritiesContains 3 RPEL repeats.
    Contains 1 SAP domain.
  • DomainThe C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MYCD antibody
    • MYCD_HUMAN antibody
    • Myocardin antibody
    • Myocd antibody

References for Anti-Myocardin antibody (ab106346)

ab106346 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (retina)
Antigen retrieval step None
Permeabilization Yes - 1% Triton
Specification retina
Blocking step normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
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Submitted Dec 09 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (pulmonary artery smooth muscle cell)
Loading amount 14 µg
Specification pulmonary artery smooth muscle cell
Treatment both treated and not treated by anti-mir145 30nM for 4 days
Gel Running Conditions Non-reduced Denaturing (10% BisTris Gel)
Blocking step Licor Blocking buffer as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 4°C
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Submitted Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"