Overview

  • Product name
  • Description
    Rabbit polyclonal to Myocardin
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal domain of Human Myocardin.

Properties

Applications

Our Abpromise guarantee covers the use of ab106346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 102 kDa.
IHC-Fr 1/50 - 1/500.

Target

  • Function
    Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
  • Tissue specificity
    Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
  • Sequence similarities
    Contains 3 RPEL repeats.
    Contains 1 SAP domain.
  • Domain
    The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MYCD antibody
    • MYCD_HUMAN antibody
    • Myocardin antibody
    • Myocd antibody

References

ab106346 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (retina)
Antigen retrieval step
None
Permeabilization
Yes - 1% Triton
Specification
retina
Blocking step
normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Dec 09 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (pulmonary artery smooth muscle cell)
Loading amount
14 µg
Specification
pulmonary artery smooth muscle cell
Treatment
both treated and not treated by anti-mir145 30nM for 4 days
Gel Running Conditions
Non-reduced Denaturing (10% BisTris Gel)
Blocking step
Licor Blocking buffer as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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