Overview

  • Product name
  • Description
    Rabbit polyclonal to Myocilin
  • Specificity
    The antibody does not cross react with any other protein in RPE cells.
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide:

    CLVWDVGARTAQLRKANDQSGR

    conjugated to KLH, corresponding to N terminal amino acids 25/46 of Myocilin

  • Positive control
    • RPE cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab41552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200.
WB 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IP 1/200.

Target

  • Function
    May participate in the obstruction of fluid outflow in the trabecular meshwork.
  • Tissue specificity
    Expressed in large amounts in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart and other tissues. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and appeared more intensively than in normal eyes, regardless of the type or clinical severity of glaucoma.
  • Involvement in disease
    Defects in MYOC are the cause of primary open angle glaucoma type 1A (GLC1A) [MIM:137750]. Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
    Defects in MYOC may also contribute to primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. Defects in MYOC may contribute to this phenotype via digenic inheritance. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early choldhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor.
  • Sequence similarities
    Contains 1 olfactomedin-like domain.
  • Post-translational
    modifications
    Different isoforms may arise by post-translational modifications.
    Glycosylated.
    Palmitoylated.
  • Cellular localization
    Rough endoplasmic reticulum. Secreted. Cell projection > cilium. Located preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells, and in the rough endoplasmic reticulum. Also secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • GLC1A antibody
    • GPOA antibody
    • JOAG antibody
    • JOAG1 antibody
    • Mutated trabecular meshwork-induced glucocorticoid response protein antibody
    • MYOC antibody
    • MYOC_HUMAN antibody
    • Myocilin antibody
    • Myocilin, trabecular meshwork inducible glucocorticoid antibody
    • TIGR antibody
    • Trabecular meshwork induced glucocorticoid response protein antibody
    • Trabecular meshwork-induced glucocorticoid response protein antibody
    see all

Anti-Myocilin antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labelling Myocilin with ab41552 at a dilution of 1/50. Counterstained with hematoxylin (blue). 40X magnigication.

References for Anti-Myocilin antibody (ab41552)

ab41552 has not yet been referenced specifically in any publications.

Product Wall

Heat-mediated antigen retrieval, Tris-EDTA pH9, 20 minutes in a steamer at ˜95-100C.  Any heating method will work: waterbath, microwave, pressure cooker.

Thank you for your patience as I have obtained our WB image for ab41552. Please find the image attached to this email. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up