Our Abpromise guarantee covers the use of ab16148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MyoD1 (ab64159). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to MyoD1 (ab64159) as Detection.|
|ELISA||Use at an assay dependent concentration.|
|IP||Use at 2 µg/mg of lysate.|
|IHC-P||Use a concentration of 2 - 4 µg/ml.|
|IHC-Fr||Use a concentration of 2 - 4 µg/ml.|
|Electron Microscopy||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.|
This image is courtesy of an anonymous Abreview
Immunohistochemical staining using ab16148 at 2ug/ml on formalin fixed, paraffin embedded samples of human rhabdomyosarcoma.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"