Anti-MyoD1 antibody [5.2F], prediluted (ab7272)

Overview

  • Product nameAnti-MyoD1 antibody [5.2F], prediluted
    See all MyoD1 primary antibodies
  • Description
    Mouse monoclonal [5.2F] to MyoD1, prediluted
  • SpecificityThis antibody is specific to a 45 kD protein. This antibody does not react with myogenin, Myf5 or Myf6. MyoD1 stains the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is expressed strongly in the tumor cell nuclei of rhabdomyosarcomas.
  • Tested applicationsSuitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    BALB/C mice were injected with a recombinant mouse MyoD1 protein.

  • EpitopeThis antibody is specific to an epitope of amino acid 3-56 in the N-terminus of mouse MyoD1.
  • Positive control
    • Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab7272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
    Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc1 antibody
    • Class C basic helix-loop-helix protein 1 antibody
    • MYF 3 antibody
    • Myf-3 antibody
    • MYF3 antibody
    • Myoblast determination protein 1 antibody
    • Myod 1 antibody
    • MYOD antibody
    • MYOD1 antibody
    • MYOD1_HUMAN antibody
    • Myogenic differentiation 1 antibody
    • Myogenic factor 3 antibody
    • Myogenic factor MYF 3 antibody
    • Myogenin D1 antibody
    • PUM antibody
    see all

References for Anti-MyoD1 antibody [5.2F], prediluted (ab7272)

ab7272 has not yet been referenced specifically in any publications.

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