Anti-MyoD1 antibody [5.8A] - BSA free (ab212662)

Overview

  • Product name
    Anti-MyoD1 antibody [5.8A] - BSA free
    See all MyoD1 primary antibodies
  • Description
    Mouse monoclonal [5.8A] to MyoD1 - BSA free
  • Specificity
    ab212662 does not cross react with myogenin, Myf5, or Myf6.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Recombinant full length protein corresponding to Mouse MyoD1.
    Database link: P10085

  • Epitope
    The epitope of ab212662 maps between amino acid 180-189 in the C terminal of mouse MyoD1 protein.
  • Positive control
    • Human rhabdomyosarcoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab212662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

for 30 minutes at room temperature.

ICC/IF Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

Final volume 100 µL.

Target

  • Function
    Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
    Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc1 antibody
    • Class C basic helix-loop-helix protein 1 antibody
    • MYF 3 antibody
    • Myf-3 antibody
    • MYF3 antibody
    • Myoblast determination protein 1 antibody
    • Myod 1 antibody
    • MYOD antibody
    • MYOD1 antibody
    • MYOD1_HUMAN antibody
    • Myogenic differentiation 1 antibody
    • Myogenic factor 3 antibody
    • Myogenic factor MYF 3 antibody
    • Myogenin D1 antibody
    • PUM antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Rhabdomyosarcoma tissue section labeling MyoD1 with ab212662 at 1 µg/mL.

References

ab212662 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Hamster Tissue sections (Muscle)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0
Permeabilization
No
Specification
Muscle
Blocking step
BSA + Milk + Serum as blocking agent for 20 minute(s) · Concentration: 2.0% · Temperature: 27°C
Fixative
Formaldehyde
Username

Dr. Marcelo Pelajo Machado

Verified customer

Submitted Jul 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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