Overview

  • Product nameAnti-MyoD1 antibodySee all MyoD1 primary antibodies ...
  • Description
    Rabbit polyclonal to MyoD1
  • Tested applicationsSandwich ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human MyoD1.

    (Peptide available as ab66473.)

  • Positive control
    • This antibody gave a positive signal in the following Tissue Lysates: Human Heart, Mouse Skeletal Muscle, Mouse Heart

Properties

Applications

Our Abpromise guarantee covers the use of ab64159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5.2F] to MyoD1 (ab16148). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [5.2F] to MyoD1 (ab16148) as Capture.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).Can be blocked with Human MyoD1 peptide (ab66473).

Target

  • FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
    Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc1 antibody
    • Class C basic helix-loop-helix protein 1 antibody
    • MYF 3 antibody
    • Myf-3 antibody
    • MYF3 antibody
    • Myoblast determination protein 1 antibody
    • Myod 1 antibody
    • MYOD antibody
    • MYOD1 antibody
    • MYOD1_HUMAN antibody
    • Myogenic differentiation 1 antibody
    • Myogenic factor 3 antibody
    • Myogenic factor MYF 3 antibody
    • Myogenin D1 antibody
    • PUM antibody
    see all

Anti-MyoD1 antibody images

  • All lanes : Anti-MyoD1 antibody (ab64159) at 1 µg/ml

    Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • ab64159 staining MyoD1 in Mouse primary adult muscle stem cells (satellite cells) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 45 minutes at 21°C. Samples were incubated with primary antibody (1/250 in 5% Goat serum + 2% BSA + 0.2% Triton X-100) for 18 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.

Protocols

References for Anti-MyoD1 antibody (ab64159)

This product has been referenced in:
  • Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). ICC/IF ; Goat . Read more (PubMed: 24699686) »
  • Nissar AA  et al. Skeletal muscle regeneration is delayed by reduction in Xin expression: consequence of impaired satellite cell activation? Am J Physiol Cell Physiol 302:C220-7 (2012). Read more (PubMed: 21975424) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Mouse Cell (Primary Adult Muscle Stem Cell (Satellite Cell))
Specification Primary Adult Muscle Stem Cell (Satellite Cell)
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde
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Submitted Oct 28 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Adipose-derived stem cells)
Specification Adipose-derived stem cells
Fixative Formaldehyde
Permeabilization Yes - Triton-X 100
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

Submitted Jan 25 2012

I am sending you a new discount code which will be valid for 1 month - for testing ab64159 in ICC/IF.

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If you don't mind, you could update the discount code in your submitted Abreview so ...

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Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Loading amount 30 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Nov 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"