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Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human MyoD1.
(Peptide available as ab66473.)
Our Abpromise guarantee covers the use of ab64159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5.2F] to MyoD1 (ab16148). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [5.2F] to MyoD1 (ab16148) as Capture.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).Can be blocked with Human MyoD1 peptide (ab66473).|
ab64159 staining MyoD1 in Mouse primary adult muscle stem cells (satellite cells) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 45 minutes at 21°C. Samples were incubated with primary antibody (1/250 in 5% Goat serum + 2% BSA + 0.2% Triton X-100) for 18 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
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