• Product nameAnti-MyoD1 antibody
    See all MyoD1 primary antibodies
  • Description
    Rabbit polyclonal to MyoD1
  • Tested applicationsSandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow, Pig, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human MyoD1.

    (Peptide available as ab66473.)

  • Positive control
    • This antibody gave a positive signal in Mouse Skeletal Muscle tissue lysate as well as the Human Rhabdomyosarcoma Cell Line Rh30.



Our Abpromise guarantee covers the use of ab64159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.1 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [5.2F] to MyoD1 (ab16148). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [5.2F] to MyoD1 (ab16148) as Capture.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).Can be blocked with Human MyoD1 peptide (ab66473).


  • FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
  • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
    Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHc1 antibody
    • Class C basic helix-loop-helix protein 1 antibody
    • MYF 3 antibody
    • Myf-3 antibody
    • MYF3 antibody
    • Myoblast determination protein 1 antibody
    • Myod 1 antibody
    • MYOD antibody
    • MYOD1 antibody
    • MYOD1_HUMAN antibody
    • Myogenic differentiation 1 antibody
    • Myogenic factor 3 antibody
    • Myogenic factor MYF 3 antibody
    • Myogenin D1 antibody
    • PUM antibody
    see all

Anti-MyoD1 antibody images

  • All lanes : Anti-MyoD1 antibody (ab64159) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate at 20 µg
    Lane 2 : Rh30 Whole Cell Lysate at 5 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 47 kDa (possible post-translational modification).

    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab64159 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    ab64159 detects a band at 45 kDa, while this differs to its predicted molecular weight of 34kDa, the banding pattern observed is consistent with what has been described in the literature PMID:19352326.

  • ab64159 staining MyoD1 in Mouse primary adult muscle stem cells (satellite cells) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 45 minutes at 21°C. Samples were incubated with primary antibody (1/250 in 5% Goat serum + 2% BSA + 0.2% Triton X-100) for 18 hours at 4°C. An Alexa Fluor® 568-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Standard Curve for Myo-D; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [5.2F] to MyoD1 (ab16148) at 5ug/ml and Detector Antibody Rabbit polyclonal to MyoD1 (ab64159) at 0.1ug/ml.


References for Anti-MyoD1 antibody (ab64159)

This product has been referenced in:
  • Ren Y  et al. Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats. PLoS One 9:e93583 (2014). ICC/IF ; Goat . Read more (PubMed: 24699686) »
  • Nissar AA  et al. Skeletal muscle regeneration is delayed by reduction in Xin expression: consequence of impaired satellite cell activation? Am J Physiol Cell Physiol 302:C220-7 (2012). Read more (PubMed: 21975424) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Sample Mouse Cell (Primary Adult Muscle Stem Cell (Satellite Cell))
Specification Primary Adult Muscle Stem Cell (Satellite Cell)
Permeabilization Yes - 0.2% Triton X-100
Fixative Paraformaldehyde

Mr. Nicholas Legendre

Verified customer

Submitted Oct 28 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Adipose-derived stem cells)
Specification Adipose-derived stem cells
Fixative Formaldehyde
Permeabilization Yes - Triton-X 100
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jan 25 2012

I am sending you a new discount code which will be valid for 1 month - for testing ab64159 in ICC/IF.

Expiration date: xxx

If you don't mind, you could update the discount code in your submitted Abreview so ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Loading amount 30 µg
Specification Skeletal muscle
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Nov 12 2010