Recombinant
RabMAb

Anti-MyoD1 antibody [EPR6653] (ab126726)

Overview

  • Product name
    Anti-MyoD1 antibody [EPR6653]
    See all MyoD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR6653] to MyoD1
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human MyoD1 (UniProt P15172).

  • Positive control
    • HeLa, A673, HT1080, and Human heart lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 34 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
    • Sequence similarities
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Post-translational
      modifications
      Acetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
      Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHc1 antibody
      • Class C basic helix-loop-helix protein 1 antibody
      • MYF 3 antibody
      • Myf-3 antibody
      • MYF3 antibody
      • Myoblast determination protein 1 antibody
      • Myod 1 antibody
      • MYOD antibody
      • MYOD1 antibody
      • MYOD1_HUMAN antibody
      • Myogenic differentiation 1 antibody
      • Myogenic factor 3 antibody
      • Myogenic factor MYF 3 antibody
      • Myogenin D1 antibody
      • PUM antibody
      see all

    Images

    • All lanes : Anti-MyoD1 antibody [EPR6653] (ab126726) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A673 cell lysate
      Lane 3 : HT1080 cell lysate
      Lane 4 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 34 kDa
    • ab126726, at 1/10 dilution staining MyoD1 in permeabilized HeLa cells by Flow Cytometry (red). A rabbit IgG negative control is shown in green.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Chen HS  et al. Benzyl butyl phthalate decreases myogenic differentiation of endometrial mesenchymal stem/stromal cells through miR-137-mediated regulation of PITX2. Sci Rep 7:186 (2017). WB . Read more (PubMed: 28298639) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (skeletal muscle (plantaris))
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    35 µg
    Specification
    skeletal muscle (plantaris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 05 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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