Overview

  • Product name
    Anti-Myofibroblasts antibody [PR 2D3]
  • Description
    Mouse monoclonal [PR 2D3] to Myofibroblasts
  • Tested applications
    Suitable for: IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation of normal colorectal mucosa (Human).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    PR 2D3
  • Myeloma
    NS0
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
  • Application notes
    IHC-Fr, Acetone Fixed: Use at an assay dependent dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      The antibody reacts with a cell membrane component of cells in the pericrypt sheath, with smooth muscle and with myofibroblasts. The gold standard for identification of myofibroblasts.

    References for Anti-Myofibroblasts antibody [PR 2D3] (ab20255)

    This product has been referenced in:
    • Kirkland SC & Henderson K Collagen IV synthesis is restricted to the enteroendocrine pathway during multilineage differentiation of human colorectal epithelial stem cells. J Cell Sci 114:2055-64 (2001). Read more (PubMed: 11493641) »
    • Richman PI  et al. Colonic pericrypt sheath cells: characterisation of cell type with new monoclonal antibody. J Clin Pathol 40:593-600 (1987). Read more (PubMed: 3301906) »

    See all 2 Publications for this product

    Product Wall

    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample
    Human Cultured Cells (lacrimal gland)
    Specification
    lacrimal gland
    Permeabilization
    Yes - 0.25% triton X-100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 16 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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