The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-Fr, Acetone Fixed: Use at an assay dependent dilution.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceThe antibody reacts with a cell membrane component of cells in the pericrypt sheath, with smooth muscle and with myofibroblasts. The gold standard for identification of myofibroblasts.
References for Anti-Myofibroblasts antibody [PR 2D3] (ab20255)
This product has been referenced in:
Kirkland SC & Henderson K Collagen IV synthesis is restricted to the enteroendocrine pathway during multilineage differentiation of human colorectal epithelial stem cells. J Cell Sci114:2055-64 (2001).
Read more (PubMed: 11493641) »
Richman PI et al. Colonic pericrypt sheath cells: characterisation of cell type with new monoclonal antibody. J Clin Pathol40:593-600 (1987).
Read more (PubMed: 3301906) »