Overview

  • Product name
  • Description
    Goat polyclonal to Myogenin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Rabbit, Goat, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide:

    C-TDAHNLHSLTSIVD

    , corresponding to C terminal amino acids 193-206 of Human Myogenin (NP_002470.2).

  • Positive control
    • Mouse Skeletal Muscle lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab103924 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 25 kDa).

Target

Images

  • Anti-Myogenin antibody (ab103924) at 2 µg/ml + Mouse Skeletal Muscle lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : ~38 kDa (why is the actual band size different from the predicted?)

References

ab103924 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (muscle precursor cells/myotubes)
Permeabilization
No
Specification
muscle precursor cells/myotubes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Aug 18 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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