Anti-Myogenin antibody (ab103924)
Key features and details
- Goat polyclonal to Myogenin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Myogenin antibody
See all Myogenin primary antibodies -
Description
Goat polyclonal to Myogenin -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Sheep, Rabbit, Goat, Cow, Dog, Human, Pig -
Immunogen
Synthetic peptide:
C-TDAHNLHSLTSIVD
, corresponding to C terminal amino acids 193-206 of Human Myogenin (NP_002470.2). -
Positive control
- Recombinant Human Myogenin protein (ab114244) can be used as a positive control in WB. Mouse Skeletal Muscle lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab103924 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 25 kDa).
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 25 kDa). A 1 hour primary incubation is recommended for this product. |
Target
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Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 281343 Cow
- Entrez Gene: 490224 Dog
- Entrez Gene: 4656 Human
- Entrez Gene: 17928 Mouse
- Entrez Gene: 497618 Pig
- Entrez Gene: 29148 Rat
- Entrez Gene: 443158 Sheep
- Omim: 159980 Human
see all -
Alternative names
- bHLHc3 antibody
- cb553 antibody
- Class C basic helix-loop-helix protein 3 antibody
see all
Images
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Anti-Myogenin antibody (ab103924) at 2 µg/ml + Mouse Skeletal Muscle lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Observed band size: ~38 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab103924 has been referenced in 2 publications.
- Rodriguez BL et al. A Comparison of Ovine Facial and Limb Muscle as a Primary Cell Source for Engineered Skeletal Muscle. Tissue Eng Part A 26:167-177 (2020). PubMed: 31469044
- Zhang W et al. Transcription factor EGR1 promotes differentiation of bovine skeletal muscle satellite cells by regulating MyoG gene expression. J Cell Physiol 233:350-362 (2018). PubMed: 28256014