Overview

  • Product nameAnti-Myogenin antibody
    See all Myogenin primary antibodies
  • Description
    Rabbit polyclonal to Myogenin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (human) conjugated to KLH

  • Positive control
    • Induced culture of E.coli (one shot Top10 competent cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab82843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/40000 is determined for one of the three peptides.
    WB: 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHc3 antibody
      • cb553 antibody
      • Class C basic helix-loop-helix protein 3 antibody
      • MYF 4 antibody
      • Myf-4 antibody
      • MYF4 antibody
      • MYOG antibody
      • MYOG_HUMAN antibody
      • Myogenic factor 4 antibody
      • Myogenic factor 4 myogenin antibody
      • Myogenin antibody
      • Myogenin myogenin factor 4 antibody
      • OTTHUMP00000039094 antibody
      see all

    Anti-Myogenin antibody images

    • All lanes : Anti-Myogenin antibody (ab82843) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa

    References for Anti-Myogenin antibody (ab82843)

    ab82843 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Skeletal Muscle)
    Loading amount 30 µg
    Specification Skeletal Muscle
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

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    Verified customer

    Submitted Nov 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"