Overview

  • Product name
  • Description
    Rabbit polyclonal to Myogenin
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (human) conjugated to KLH

  • Positive control
    • Induced culture of E.coli (one shot Top10 competent cells).

Properties

Applications

Our Abpromise guarantee covers the use of ab82843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/40000 is determined for one of the three peptides.
    WB: 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
    • Sequence similarities
      Contains 1 basic helix-loop-helix (bHLH) domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • bHLHc3 antibody
      • cb553 antibody
      • Class C basic helix-loop-helix protein 3 antibody
      • MYF 4 antibody
      • Myf-4 antibody
      • MYF4 antibody
      • MYOG antibody
      • MYOG_HUMAN antibody
      • Myogenic factor 4 antibody
      • Myogenic factor 4 myogenin antibody
      • Myogenin antibody
      • Myogenin myogenin factor 4 antibody
      • OTTHUMP00000039094 antibody
      see all

    Anti-Myogenin antibody images

    • All lanes : Anti-Myogenin antibody (ab82843) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 39 kDa
      Observed band size : 39 kDa

    References for Anti-Myogenin antibody (ab82843)

    ab82843 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rabbit Tissue lysate - whole (Muscle (gastrocnemius))
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    35 µg
    Specification
    Muscle (gastrocnemius)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Ms. Sandra Pérez

    Verified customer

    Submitted Apr 20 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skeletal Muscle)
    Loading amount
    30 µg
    Specification
    Skeletal Muscle
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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