Overview

  • Product nameAnti-Myogenin antibody [EPR4789]
    See all Myogenin primary antibodies
  • Description
    Rabbit monoclonal [EPR4789] to Myogenin
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Myogenin.

  • Positive control
    • C2C12 and Human fetal muscle lysates; Human rhabdomyosarcoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IP.
  • Target

    Anti-Myogenin antibody [EPR4789] images

    • All lanes : Anti-Myogenin antibody [EPR4789] (ab124800) at 1/1000 dilution

      Lane 1 : C2C12 lysates
      Lane 2 : Human fetal muscle lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 25 kDa
    • ab124800, at a 1/100 dilution, staining Myogenin in paraffin embedded Human rhabdomyosarcoma tissue by Immunohistochemistry.

    References for Anti-Myogenin antibody [EPR4789] (ab124800)

    This product has been referenced in:
    • Smith IJ  et al. Inhibition of Janus kinase signaling during controlled mechanical ventilation prevents ventilation-induced diaphragm dysfunction. FASEB J 28:2790-803 (2014). WB ; Rat . Read more (PubMed: 24671708) »
    • Yu Y  et al. Kindlin 2 regulates myogenic related factor myogenin via a canonical Wnt signaling in myogenic differentiation. PLoS One 8:e63490 (2013). WB ; Mouse . Read more (PubMed: 23717433) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"