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Product name Anti-Myogenin antibody [EPR4789] See all Myogenin primary antibodies
Rabbit monoclonal [EPR4789] to Myogenin
Tested applications Suitable for:
WB, IHC-P, Flow Cyt more details Unsuitable for:
Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human Myogenin.
C2C12 and Human fetal muscle lysates; Human rhabdomyosarcoma tissue.
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb
® technology. RabMAb ® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity Tissue culture supernatant
Clone number EPR4789
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 25 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/10 - 1/100.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notes Is unsuitable for IP.
Function Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Sequence similarities Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization Nucleus.
Information by UniProt
Class C basic helix-loop-helix protein 3 antibody
Anti-Myogenin antibody [EPR4789] images
Western blot - Anti-Myogenin antibody [EPR4789] (ab124800)
All lanes : Anti-Myogenin antibody [EPR4789] (ab124800) at 1/1000 dilution Lane 1 : C2C12 lysates Lane 2 : Human fetal muscle lysates Lysates/proteins at 10 µg per lane. Secondary Goat anti-Rabbit HRP at 1/2000 dilution Predicted band size : 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (ab124800)
ab124800, at a 1/100 dilution, staining Myogenin in paraffin embedded Human rhabdomyosarcoma tissue by Immunohistochemistry.
References for Anti-Myogenin antibody [EPR4789] (ab124800)
This product has been referenced in:
et al. Inhibition of Janus kinase signaling during controlled mechanical ventilation prevents ventilation-induced diaphragm dysfunction. FASEB J 28:2790-803 (2014).
Read more (PubMed: 24671708) »
et al. Kindlin 2 regulates myogenic related factor myogenin via a canonical Wnt signaling in myogenic differentiation. PLoS One 8:e63490 (2013).
Read more (PubMed: 23717433) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"