Overview

  • Product nameAnti-Myogenin antibody [F12B]
    See all Myogenin primary antibodies
  • Description
    Mouse monoclonal [F12B] to Myogenin
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 30 - 224 of Rat Myogenin.

  • EpitopeAmino acids 158-178
  • Positive control
    • Rh30 cells. Rhabdomyosarcoma or Wilm’s tumors.

Properties

Applications

Our Abpromise guarantee covers the use of ab80956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 25 kDa. Use at recommended concentrations for 2hrs at RT.
IP Use at 2 µg/mg of lysate.
IHC-P Use a concentration of 1 - 2 µg/ml. Use at recommended concentrations for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
ICC/IF Use at an assay dependent concentration.
EMSA Use at an assay dependent concentration.

Target

Anti-Myogenin antibody [F12B] images

  • ab80956, at 1 µg/ml, staining Myogenin in formalin-fixed, paraffin-embedded human rhabdomyosarcoma by immunohistochemistry, using a peroxidase-conjugated secondary and AEC chromogen. Note nuclear staining of tumor cells.

  • Anti-Myogenin antibody [F12B] (ab80956) at 1 µg/ml + Rh30 cells

    Predicted band size : 25 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

References for Anti-Myogenin antibody [F12B] (ab80956)

This product has been referenced in:
  • Wright WE  et al. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interactions. Dev Genet 19:131-8 (1996). Read more (PubMed: 8900045) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"