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BALB/ C mice were injected with recombinant protein containing rat myogenin amino acid 30 - 224.
Our Abpromise guarantee covers the use of ab1835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Gel supershift assays||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Recommended starting point for ab1835 incubation is 30min RT.|
|IP||Use at an assay dependent concentration.|
|WB||1/250. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).
Abcam recommends using BSA as the blocking agent.
|IHC-FoFr||Use at an assay dependent concentration.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab1835 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Ruma Raha-Chowdhury, University Of Cambridge, United KingdomNon-specific bands detected 37, 64, 74, 95kDa
ab1835 - immunohistochemistry
Formalin fixed paraffin embedded human rhabdomyosarcoma stained with Myogenin using ABC and AEC chromogen.
ab1835 at 1/50 dilution staining pig differentiated skeletal myoblast cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized with Triton-X100 and incubated with the antibody overnight at 4C. An Alexa Fluor 488 conjugated goat anti-mouse antibody was used as the secondary. The image was captured using a confocal laser scanning microscope with an additional Differential Interference Contrast (DIC) mode. The image shows DAPI nuclear counterstain (blue, upper left panel), Myogenin staining (green, upper right panel), DIC (phase) image (lower left panel) and overlay (lower right panel).
Myogenin is not expressed in proliferating myoblasts, but rather in mononucleated differentiating myoblasts (See abreview for more detail).
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