Anti-Myogenin antibody [MGN185 + F5D] (ab199017)
Key features and details
- Mouse monoclonal [MGN185 + F5D] to Myogenin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Myogenin antibody [MGN185 + F5D]
See all Myogenin primary antibodies -
Description
Mouse monoclonal [MGN185 + F5D] to Myogenin -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cat, Pig -
Immunogen
Recombinant fragment corresponding to Myogenin aa 1-250. Human myogenin recombinant protein (P15173) (Clone: MGN185); Rat Myogenin recombinant fragment containing amino acid 30-224 (Protein length = 287) (P20428) (Clone: F5D).
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Positive control
- IHC-P: Human rhabdomyosarcoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab199017 was purified from Bioreactor Concentrate. -
Clonality
Monoclonal -
Clone number
MGN185 + F5D -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab199017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Perform antigen retrieval for 45 min at 95°C followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Perform antigen retrieval for 45 min at 95°C followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 30 minutes at room temperature. |
Target
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Function
Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 101100276 Cat
- Entrez Gene: 4656 Human
- Entrez Gene: 17928 Mouse
- Entrez Gene: 497618 Pig
- Entrez Gene: 29148 Rat
- Omim: 159980 Human
- SwissProt: P15173 Human
- SwissProt: P12979 Mouse
see all -
Alternative names
- bHLHc3 antibody
- cb553 antibody
- Class C basic helix-loop-helix protein 3 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab199017 has been referenced in 2 publications.
- Osaki T et al. On-chip 3D neuromuscular model for drug screening and precision medicine in neuromuscular disease. Nat Protoc 15:421-449 (2020). PubMed: 31932771
- Boularaoui SM et al. Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle. J Tissue Eng Regen Med 12:e918-e936 (2018). PubMed: 28101909