Overview

  • Product nameAnti-Myoglobin antibody [4E2]
    See all Myoglobin primary antibodies
  • Description
    Mouse monoclonal [4E2] to Myoglobin
  • Tested applicationsSuitable for: WB, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human cardiac myoglobin

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab8343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [7C3] to Myoglobin (HRP) (ab8345). Can be used as capture antibody in conjunction with recommended pair.

Target

Anti-Myoglobin antibody [4E2] images

  • All lanes : Anti-Myoglobin antibody [4E2] (ab8343) at 5 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 18 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

References for Anti-Myoglobin antibody [4E2] (ab8343)

This product has been referenced in:
  • Flonta SE  et al. Expression and functional regulation of myoglobin in epithelial cancers. Am J Pathol 175:201-6 (2009). WB ; Human . Read more (PubMed: 19541931) »

See 1 Publication for this product

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Thank you for your inquiry.

I can confirm that this antibody was produced by immunizing with full length human cardiac myoglobin.

I hope this information is helpful and wish your customer good luck with their research.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"