Overview

  • Product nameAnti-Myoglobin antibody [6H8B5]
    See all Myoglobin primary antibodies
  • Description
    Mouse monoclonal [6H8B5] to Myoglobin
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Ni-NTA purified truncated recombinant Myoglobin expressed in E. Coli strain BL21 (DE3)

  • Positive control
    • Human skeletal muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab47702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 17 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/2000.
ELISA 1/10000.

Target

Anti-Myoglobin antibody [6H8B5] images

  • ab47702 staining Myoglobin in cow skeletal muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed by heat fixation and blocked with 5% serum for 30 minutes at 23°C. Samples were incubated with primary antibody (1/2000) for 1 hour at 23°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Green - dystrophin, Blue - Hoechst.

    See Abreview

  • Anti-Myoglobin antibody [6H8B5] (ab47702) + Truncated Myoglobin recombinant protein (Tagged)

    Predicted band size : 17 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • Ab47702 staining human Myoglobin in human skeletal muscle tissue, showing cytoplasmic localization, with DAB staining.

References for Anti-Myoglobin antibody [6H8B5] (ab47702)

This product has been referenced in:
  • Perrone CE  et al. Methionine restriction effects on mitochondrial biogenesis and aerobic capacity in white adipose tissue, liver, and skeletal muscle of F344 rats. Metabolism 59:1000-11 (2010). WB ; Rat . Read more (PubMed: 20045141) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Cow Cell (Skeletal Muscle)
Specification Skeletal Muscle
Permeabilization No
Fixative Heat Fix
Username

Dr. John Gonzalez

Verified customer

Submitted May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"