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Ni-NTA purified truncated recombinant Myoglobin expressed in E. Coli strain BL21 (DE3)
Our Abpromise guarantee covers the use of ab47702 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 39 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/500 - 1/2000.|
ab47702 staining Myoglobin in cow skeletal muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed by heat fixation and blocked with 5% serum for 30 minutes at 23°C. Samples were incubated with primary antibody (1/2000) for 1 hour at 23°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Green - dystrophin, Blue - Hoechst.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"