Overview

  • Product nameAnti-Myoglobin antibody
    See all Myoglobin primary antibodies
  • Description
    Mouse polyclonal to Myoglobin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity

    Predicted to work with: Dog, Human, Pig, Chimpanzee
  • Immunogen

    Recombinant fusion protein: MGLSDGEWHL VLNVWGKVET DLAGHGQEVL IRLFKSHPET LEKFDKFKHL KSEDDMRRSE DLRKHGNTVL TALGGILKKK GHHEAELKPL AQSHATKHKI , corresponding to amino acids 1-100 of Grey seal (Halichoerus grypus) Myoglobin

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 17 kDa.

Target

Anti-Myoglobin antibody images

  • All lanes : Anti-Myoglobin antibody (ab43127) at 1/1000 dilution

    Lane 1 : Total protein extract from E.coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen
    Lane 2 : Total protein extract from E.coli with ~50ng to 500ng of the antigen fusion protein

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa

References for Anti-Myoglobin antibody (ab43127)

This product has been referenced in:
  • Vázquez-Medina JP  et al. Apnea stimulates the adaptive response to oxidative stress in elephant seal pups. J Exp Biol 214:4193-200 (2011). WB . Read more (PubMed: 22116762) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"