Overview

  • Product nameAnti-Myoglobin antibody
    See all Myoglobin primary antibodies
  • Description
    Rabbit polyclonal to Myoglobin
  • Tested applicationsSuitable for: WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human Myoglobin.

    (Peptide available as ab88944.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Skeletal Muscle; Human Heart.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab78441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [BDI927] to Myoglobin (ab19607). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [BDI927] to Myoglobin (ab19607) as Capture.

Target

Anti-Myoglobin antibody images

  • Standard Curve for Myoglobin (Analyte: Myoglobin protein (ab96036)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [BDI927] to Myoglobin (ab19607) at 5µg/ml and Detector Antibody Rabbit polyclonal to Myoglobin (ab78441) at 0.5µg/ml.
  • All lanes : Anti-Myoglobin antibody (ab78441) at 0.5 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa


    Exposure time : 10 seconds
  • All lanes : Anti-Myoglobin antibody (ab78441) at 0.5 µg/ml

    Lane 1 : Natural Human Myoglobin protein (ab77876) at 0.1 µg
    Lane 2 : Natural Human Myoglobin protein (ab77876) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

Protocols

References for Anti-Myoglobin antibody (ab78441)

ab78441 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"