Overview

  • Product nameAnti-Myoglobin antibody
    See all Myoglobin primary antibodies
  • Description
    Rabbit polyclonal to Myoglobin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Myoglobin.

    (Peptide available as ab90994.)

  • Positive control
    • This antibody gave a positive signal in Human Heart; Human Skeletal Muscle.

Properties

Applications

Our Abpromise guarantee covers the use of ab88055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Target

Anti-Myoglobin antibody images

  • All lanes : Anti-Myoglobin antibody (ab88055) at 0.5 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 5 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution (Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP))
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 30 kDa,48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 seconds

References for Anti-Myoglobin antibody (ab88055)

ab88055 has not yet been referenced specifically in any publications.

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