Anti-Myoglobin antibody [EP3081Y] (ab77232)

Overview

  • Product nameAnti-Myoglobin antibody [EP3081Y]
    See all Myoglobin primary antibodies
  • Description
    Rabbit monoclonal [EP3081Y] to Myoglobin
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues on the C terminus of human Myoglobin

  • Positive control
    • Human, rat and mouse muscle tissue lysates; human heart muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:
    Anti-Myoglobin antibody (HRP) [EP3081Y] (ab199243)

Properties

Applications

Our Abpromise guarantee covers the use of ab77232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IP 1/40.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use of an HRP/AP polymerized secondary antibody is recommended.

ICC/IF Use at an assay dependent concentration. PubMed: 24466343

Target

Anti-Myoglobin antibody [EP3081Y] images

  • ab77232 showing positive staining in Skeletal muscle tissue.



  • Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    Western blot analysis of Pig skeletal muscle whole tissue lysate (10μg/lane) labelling Myoglobin with ab77232 at 1/5000 in 1x PBS + 0.1% Tween-20 for 17 hours at 4ºC. A IRDye® 680-conjugated Donkey anti-rabbit polyclonal (1/40000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Myoglobin antibody [EP3081Y] (ab77232) at 1/5000 dilution

    Lane 1 : Human heart muscle lysate
    Lane 2 : Mouse heart muscle lysate
    Lane 3 : Rat heart muscle lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
  • ab77232, at a 1/250 dilution, staining Myoglobin in paraffin embedded human heart muscle by Immunohistochemistry.
  • ab77232 showing negative staining in Normal brain tissue.

  • ab77232 showing negative staining in Normal kidney tissue.

  • ab77232 showing negative staining in Normal liver tissue.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-Myoglobin antibody [EP3081Y] (ab77232)

This product has been referenced in:
  • He N  et al. Isolation and characterization of hair follicle stem cells from Arbas Cashmere goat. Cytotechnology 68:2579-2588 (2016). Read more (PubMed: 27193423) »
  • Galicia-Vázquez G  et al. Regulation of eukaryotic initiation factor 4AII by MyoD during murine myogenic cell differentiation. PLoS One 9:e87237 (2014). ICC/IF ; Mouse . Read more (PubMed: 24466343) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (14% 37.5:1)
Sample Pig Tissue lysate - whole (Skeletal Muscle)
Specification Skeletal Muscle
Blocking step Thermo Starting Block 37542 as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Sep 16 2013

Thank you for contacting us.

The IgG concentration ab77232, lot GR52810, is 0.100 mg/mL.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"