A synthetic peptide corresponding to residues on the C terminus of human Myoglobin
Our Abpromise guarantee covers the use of ab77232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 17 kDa.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Use of an HRP/AP polymerized secondary antibody is recommended.
|ICC/IF||Use at an assay dependent concentration. PubMed: 24466343|
This image is courtesy of an anonymous Abreview
Western blot analysis of Pig skeletal muscle whole tissue lysate (10μg/lane) labelling Myoglobin with ab77232 at 1/5000 in 1x PBS + 0.1% Tween-20 for 17 hours at 4ºC. A IRDye® 680-conjugated Donkey anti-rabbit polyclonal (1/40000) was used as the secondary antibody.