Anti-MYOM3 antibody (ab121973)
Key features and details
- Rabbit polyclonal to MYOM3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MYOM3 antibody -
Description
Rabbit polyclonal to MYOM3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen: ASLKVSCTYK EDEGLYMVRV PSPFGPREQS TYVLVRDAEA ENPGAPGSPL NVRCLDVNRD CLILTWAPPS DTRGNPITAY TIERCQGESG EWI, corresponding to amino acids 328-420 of Human MYOM3 (UniProt Q5VTT5).
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Positive control
- Human Plasma lysate, Human small intestine tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/250 - 1/500.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
Target
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Sequence similarities
Contains 5 fibronectin type-III domains.
Contains 4 Ig-like C2-type (immunoglobulin-like) domains. - Information by UniProt
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Database links
- Entrez Gene: 127294 Human
- SwissProt: Q5VTT5 Human
- Unigene: 523413 Human
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Alternative names
- MYOM3 antibody
- MYOM3_HUMAN antibody
- Myomesin family member 3 antibody
see all
Images
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All lanes : Anti-MYOM3 antibody (ab121973) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate -
Immunofluorescent staining of Human cell line A-431 shows positivity in vesicles. Recommended concentration of ab121973 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121973 at 1/20 dilution staining MYOM3 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121973 has not yet been referenced specifically in any publications.