antigen sequence: CRCPIQGLVE GQSYRFRVRA ISRVGSSVPS KASELVVMGD HDAARRKTEI PFDLGNKITI STDAFEDTVT IPSPPTNVHA SEI, corresponding to amino acids 430-512 of Human MYOM3.
Our Abpromise guarantee covers the use of ab122081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunocytochemistry/Immunofluorescence analysis of A-431 cells labelling MYOM3 with ab122081 shows positivity in the cytoplasm & nucleus but is excluded from the nucleoli.
ab122081 has not yet been referenced specifically in any publications.