Overview

  • Product name
  • Description
    Rabbit polyclonal to Myosin 1C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protien fragment, corresponding to a region within amino acids 1-1028 of Human Myosin 1C isoform 2 (UniProt ID: O00159-2).

  • Positive control
    • A431 cell lysate; Myosin 1C-transfected 293T whole cell Lysate; Human PC14 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 118 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function
    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. Component of the hair cell's (the sensory cells of the inner ear) adaptation-motor complex. Acts as a mediator of adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells. Binds phosphoinositides and links the actin cytoskeleton to cellular membranes.
    Isoform 3 is involved in regulation of transcription. Associated with transcriptional active ribosomal genes. Appears to cooperate with the WICH chromatin-remodeling complex to facilitate transcription. Necessary for the formation of the first phosphodiester bond during transcription initiation.
  • Sequence similarities
    Contains 2 IQ domains.
    Contains 1 myosin head-like domain.
  • Domain
    Binds directly to large unilamellar vesicles (LUVs) containing phosphatidylinositol 4,5-bisphosphate (PIP2) or inositol 1,4,5-trisphosphate (InsP3). The PIP2-binding site corresponds to a putative PH domain present in its tail domain.
  • Cellular localization
    Cytoplasm. Cell membrane. Cell projection > stereocilium membrane. Colocalizes with CABP1 and CIB1 at cell margin, membrane ruffles and punctate regions on the cell membrane. Colocalizes in adipocytes with GLUT4 in actin-based membranes. Localizes transiently at cell membrane to region known to be enriched in PIP2. Activation of phospholipase C results in its redistribution to the cytoplasm and Nucleus > nucleoplasm. Nucleus > nucleolus. Nucleus > nuclear pore complex. Colocalizes with RNA polymerase II in the nucleus. Colocalizes with RNA polymerase I in nucleoli (By similarity). In the nucleolus, is localized predominantly in dense fibrillar component (DFC) and in granular component (GC). Accumulates strongly in DFC and GC during activation of transcription. Colocalizes with transcription sites. Colocalizes in the granular cortex at the periphery of the nucleolus with RPS6. Colocalizes in nucleoplasm with RPS6 and actin that are in contact with RNP particles. Colocalizes with RPS6 at the nuclear pore level.
  • Information by UniProt
  • Database links
  • Alternative names
    • MMI beta antibody
    • MMI-beta antibody
    • MMIb antibody
    • MYO1C antibody
    • MYO1C_HUMAN antibody
    • Myosin I beta antibody
    • Myosin Ic antibody
    • Myosin-Ic antibody
    • MYR2 antibody
    • NMI antibody
    • Nuclear myosin I antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human PC14 xenograft tissue, labeling Myosin 1C using ab154498 at a 1/500 dilution.
  • All lanes : Anti-Myosin 1C antibody (ab154498) at 1/5000 dilution

    Lane 1 : Non-transfected 293T whole cell lysate
    Lane 2 : Myosin 1C-transfected 293T whole cell Lysate

    Predicted band size: 118 kDa



    5% SDS PAGE
  • Anti-Myosin 1C antibody (ab154498) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 118 kDa



    5% SDS PAGE

References

ab154498 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Mouse Cell (mouse embryo E0.5)
Specification
mouse embryo E0.5
Permeabilization
Yes - Triton X100 0.1%
Fixative
Paraformaldehyde
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Submitted Jun 25 2013

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