Anti-Myosin 1C antibody - Carboxyterminal end (ab71486)

Overview

  • Product nameAnti-Myosin 1C antibody - Carboxyterminal end
    See all Myosin 1C primary antibodies
  • Description
    Rabbit polyclonal to Myosin 1C - Carboxyterminal end
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Positive control
    • K562 cell lysate, human prostate carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).
IHC-P 1/10 - 1/50.
ELISA 1/1000.

Target

  • FunctionMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. Component of the hair cell's (the sensory cells of the inner ear) adaptation-motor complex. Acts as a mediator of adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells. Binds phosphoinositides and links the actin cytoskeleton to cellular membranes.
    Isoform 3 is involved in regulation of transcription. Associated with transcriptional active ribosomal genes. Appears to cooperate with the WICH chromatin-remodeling complex to facilitate transcription. Necessary for the formation of the first phosphodiester bond during transcription initiation.
  • Sequence similaritiesContains 2 IQ domains.
    Contains 1 myosin head-like domain.
  • DomainBinds directly to large unilamellar vesicles (LUVs) containing phosphatidylinositol 4,5-bisphosphate (PIP2) or inositol 1,4,5-trisphosphate (InsP3). The PIP2-binding site corresponds to a putative PH domain present in its tail domain.
  • Cellular localizationCytoplasm. Cell membrane. Cell projection > stereocilium membrane. Colocalizes with CABP1 and CIB1 at cell margin, membrane ruffles and punctate regions on the cell membrane. Colocalizes in adipocytes with GLUT4 in actin-based membranes. Localizes transiently at cell membrane to region known to be enriched in PIP2. Activation of phospholipase C results in its redistribution to the cytoplasm and Nucleus > nucleoplasm. Nucleus > nucleolus. Nucleus > nuclear pore complex. Colocalizes with RNA polymerase II in the nucleus. Colocalizes with RNA polymerase I in nucleoli (By similarity). In the nucleolus, is localized predominantly in dense fibrillar component (DFC) and in granular component (GC). Accumulates strongly in DFC and GC during activation of transcription. Colocalizes with transcription sites. Colocalizes in the granular cortex at the periphery of the nucleolus with RPS6. Colocalizes in nucleoplasm with RPS6 and actin that are in contact with RNP particles. Colocalizes with RPS6 at the nuclear pore level.
  • Information by UniProt
  • Database links
  • Alternative names
    • MMI beta antibody
    • MMI-beta antibody
    • MMIb antibody
    • MYO1C antibody
    • MYO1C_HUMAN antibody
    • Myosin I beta antibody
    • Myosin Ic antibody
    • Myosin-Ic antibody
    • MYR2 antibody
    • NMI antibody
    • Nuclear myosin I antibody
    see all

Anti-Myosin 1C antibody - Carboxyterminal end images

  • Anti-Myosin 1C antibody - Carboxyterminal end (ab71486) at 1/60 dilution + K562 cell lysate at 35 µg

    Predicted band size : 118 kDa
    Observed band size : 118 kDa
    Additional bands at : multiple kDa. We are unsure as to the identity of these extra bands.
  • Human prostate carcinoma tissue with ab71486 at 1/10 dilution. DAB staining.
  • All lanes : Anti-Myosin 1C antibody - Carboxyterminal end (ab71486) at 1/1000 dilution

    Lane 1 : Human 293T whole cell lysate
    Lane 2 : Human 293T whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG at 1/40000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 118 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Myosin 1C antibody - Carboxyterminal end (ab71486)

ab71486 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T cell)
Loading amount 50 µg
Specification 293T cell
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"