Recombinant
RabMAb

Anti-Myosin 1C antibody [EPR14771] (ab194828)

Overview

  • Product name
    Anti-Myosin 1C antibody [EPR14771]
    See all Myosin 1C primary antibodies
  • Description
    Rabbit monoclonal [EPR14771] to Myosin 1C
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Myosin 1C aa 850-950. The exact sequence is proprietary.
    Database link: O00159

  • Positive control
    • A431, HT1080, 293T cell lysates; mouse heart and rat heart lysates; Human tonsil tissue; A431 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14771
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab194828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/80.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
ICC/IF 1/100.

Target

  • Function
    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. Component of the hair cell's (the sensory cells of the inner ear) adaptation-motor complex. Acts as a mediator of adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells. Binds phosphoinositides and links the actin cytoskeleton to cellular membranes.
    Isoform 3 is involved in regulation of transcription. Associated with transcriptional active ribosomal genes. Appears to cooperate with the WICH chromatin-remodeling complex to facilitate transcription. Necessary for the formation of the first phosphodiester bond during transcription initiation.
  • Sequence similarities
    Contains 2 IQ domains.
    Contains 1 myosin head-like domain.
  • Domain
    Binds directly to large unilamellar vesicles (LUVs) containing phosphatidylinositol 4,5-bisphosphate (PIP2) or inositol 1,4,5-trisphosphate (InsP3). The PIP2-binding site corresponds to a putative PH domain present in its tail domain.
  • Cellular localization
    Cytoplasm. Cell membrane. Cell projection > stereocilium membrane. Colocalizes with CABP1 and CIB1 at cell margin, membrane ruffles and punctate regions on the cell membrane. Colocalizes in adipocytes with GLUT4 in actin-based membranes. Localizes transiently at cell membrane to region known to be enriched in PIP2. Activation of phospholipase C results in its redistribution to the cytoplasm and Nucleus > nucleoplasm. Nucleus > nucleolus. Nucleus > nuclear pore complex. Colocalizes with RNA polymerase II in the nucleus. Colocalizes with RNA polymerase I in nucleoli (By similarity). In the nucleolus, is localized predominantly in dense fibrillar component (DFC) and in granular component (GC). Accumulates strongly in DFC and GC during activation of transcription. Colocalizes with transcription sites. Colocalizes in the granular cortex at the periphery of the nucleolus with RPS6. Colocalizes in nucleoplasm with RPS6 and actin that are in contact with RNP particles. Colocalizes with RPS6 at the nuclear pore level.
  • Information by UniProt
  • Database links
  • Alternative names
    • MMI beta antibody
    • MMI-beta antibody
    • MMIb antibody
    • MYO1C antibody
    • MYO1C_HUMAN antibody
    • Myosin I beta antibody
    • Myosin Ic antibody
    • Myosin-Ic antibody
    • MYR2 antibody
    • NMI antibody
    • Nuclear myosin I antibody
    see all

Images

  • All lanes : Anti-Myosin 1C antibody [EPR14771] (ab194828) at 1/20000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HT1080 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 122 kDa

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Myosin 1C with ab194828 at 1/200 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194828. Cytoplasm staining on Human tonsil was observed.

  • Immunofluorescent analysis of A431 cells labeling Myosin 1C with ab194828 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm and nuclear on A431 cell line was observed.

  • All lanes : Anti-Myosin 1C antibody [EPR14771] (ab194828) at 1/2000 dilution

    Lane 1 : Mouse heart lysate
    Lane 2 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 122 kDa

  • Flow cytometry analysis of HeLa cells labelling Myosin 1C (red) with purified ab194828 at dilution of 1/80. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

References

ab194828 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, Mel202, A431, HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HeLa, Mel202, A431, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Jul 22 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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