Overview

  • Product nameAnti-Myosin 1G antibody
  • Description
    Rabbit polyclonal to Myosin 1G
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 271-356 (SVHRILAAIL HLGNIEFVET EEGGLQKEGL AVAEEALVDH VAELTATPRD LVLRSLLART VASGGRELIE KGHTAAEASY ARDACA) of Human Myosin 1G.

  • Positive control
    • Human lymph node tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab121657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMyosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments.
    Precursor of the minor histocompatibility antigen HA-2. More generally, minor histocompatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and their expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocompatibility antigen in HLA-matched sibling marrow transplants. HA-2 is restricted to MHC class I HLA-A*0201.
  • Tissue specificitySpecifically expressed in hematopoietic cells.
  • Sequence similaritiesContains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Cellular localizationCell membrane. Localization at the membrane is not highly dependent on phosphatidylinositol 4,5-bisphosphate levels. Released from the membrane in the presence of ATP. May be enriched in peripheral processes, such as microvilli or ruffles.
  • Information by UniProt
  • Database links
  • Alternative names
    • mHag HA-2 antibody
    • Minor histocompatibility antigen HA-2 antibody
    • Myo1g antibody
    • MYO1G_HUMAN antibody
    • myosin IG antibody
    see all

Anti-Myosin 1G antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121657 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121657, at 1/150 dilution, staining Myosin 1G in paraffin-embedded Human lymph node tissue by Immunohistochemistry.

References for Anti-Myosin 1G antibody (ab121657)

ab121657 has not yet been referenced specifically in any publications.

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