Anti-Myosin antibody [414] (ab8070)


  • Product nameAnti-Myosin antibody [414]
    See all Myosin primary antibodies
  • Description
    Mouse monoclonal [414] to Myosin
  • SpecificityThis monoclonal antibody reacts in immunoblots specific with myosin and is specific to the 75kD of Myosin. Does not react with alpha-cardiac myosin. Does not react with the light-chain but with the last 75 kD of the myosin molecule. These 75 kD are located on a myosin-common site-chain.
  • Tested applicationsSuitable for: IHC-Fr, ICC, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Chicken
  • Positive control
    • Chicken muscle myomyofibrils.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.1% Sodium azide
    Constituent: 1% BSA
  • PurityTissue culture supernatant
  • Primary antibody notesThis antibody, directed against myosin can be used for detection of myosin in frozen sections as well as in cultured cells. Applicable in paraffin sections only with microwave oven pretreatment. Also works in immunoblotting.
  • ClonalityMonoclonal
  • Clone number414
  • IsotypeIgM
  • Light chain typeunknown
  • Research areas


Our Abpromise guarantee covers the use of ab8070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris.

WB Use at an assay dependent concentration.


  • FunctionMuscle contraction.
  • Sequence similaritiesContains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Alternative names
    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MYH2 antibody
    • MYH3 antibody
    • MYH4 antibody
    • MYH6 antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • MYL1 antibody
    • MYL2 antibody
    • MYL3 antibody
    • MYL5 antibody
    • MYL6 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin 1 antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 1 skeletal muscle adult antibody
    • Myosin heavy chain 2 skeletal muscle adult antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain 3 skeletal muscle adult antibody
    • Myosin heavy chain 4 skeletal muscle adult antibody
    • Myosin heavy chain 6 skeletal muscle adult antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin heavy chain skeletal muscle adult 1 antibody
    • Myosin II antibody
    • Myosin light chain 1 alkali skeletal fast antibody
    • Myosin light chain 3 alkali ventricular skeletal slow antibody
    • Myosin light chain 4 alkali atrial embryonic antibody
    • Myosin light chain 5 regulatory antibody
    • Myosin light chain 6 alkali smooth muscle and non muscle antibody
    • Myosin-1 antibody
    • Myosin1 antibody
    • skeletal muscle antibody
    see all

References for Anti-Myosin antibody [414] (ab8070)

ab8070 has not yet been referenced specifically in any publications.

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Thank you for responding so promptly. I'm sorry to hear you're not happy with my offer. At Abcam we value each and every one of our customers. However, I must stand by our policy. We do not routinely offer free or trial sized samples for testing purpo...

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Thank you for your inquiry. Yes, the antibody can be used for smoothe muscle.

Thanks you for your message. The deadline for placing orders to be dispatched before the holidays is midday December 18th. Please do let me know how soon you need the products. Would it be all right at the beginning of January? Or is it too late? ...

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This antibody will react with muscle and heart tissue, however it will not react with alpha-cardiac myosin. Furthermore this antibody has only been tested on human tissue and we suspect that it will not cross-react with mouse and/or rat although this h...

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All the information we have for antibodies to myosin, (ab7784, ab7829, ab7786, ab7785,) is in the datasheets