Anti-Myosin antibody [414] (ab8070)

Overview

  • Product name
    Anti-Myosin antibody [414]
    See all Myosin primary antibodies
  • Description
    Mouse monoclonal [414] to Myosin
  • Specificity
    This monoclonal antibody reacts in immunoblots specific with myosin and is specific to the 75kD of Myosin. Does not react with alpha-cardiac myosin. Does not react with the light-chain but with the last 75 kD of the myosin molecule. These 75 kD are located on a myosin-common site-chain.
  • Tested applications
    Suitable for: IHC-Fr, ICC, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Chicken
  • Positive control
    • Chicken muscle myomyofibrils.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: 1% BSA
  • Purity
    Tissue culture supernatant
  • Primary antibody notes
    This antibody, directed against myosin can be used for detection of myosin in frozen sections as well as in cultured cells. Applicable in paraffin sections only with microwave oven pretreatment. Also works in immunoblotting.
  • Clonality
    Monoclonal
  • Clone number
    414
  • Isotype
    IgM
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Use at 1/5 - 1/10.(Only with microwave oven pretreatment. Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher). Dilute immediately before use with PBS or Tris.

WB Use at an assay dependent concentration.

Target

  • Function
    Muscle contraction.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization
    Cytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Alternative names
    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MYH2 antibody
    • MYH3 antibody
    • MYH4 antibody
    • MYH6 antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • MYL1 antibody
    • MYL2 antibody
    • MYL3 antibody
    • MYL5 antibody
    • MYL6 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin 1 antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 1 skeletal muscle adult antibody
    • Myosin heavy chain 2 skeletal muscle adult antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain 3 skeletal muscle adult antibody
    • Myosin heavy chain 4 skeletal muscle adult antibody
    • Myosin heavy chain 6 skeletal muscle adult antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin heavy chain skeletal muscle adult 1 antibody
    • Myosin II antibody
    • Myosin light chain 1 alkali skeletal fast antibody
    • Myosin light chain 3 alkali ventricular skeletal slow antibody
    • Myosin light chain 4 alkali atrial embryonic antibody
    • Myosin light chain 5 regulatory antibody
    • Myosin light chain 6 alkali smooth muscle and non muscle antibody
    • Myosin-1 antibody
    • Myosin1 antibody
    • skeletal muscle antibody
    see all

References

ab8070 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for responding so promptly. I'm sorry to hear you're not happy with my offer. At Abcam we value each and every one of our customers. However, I must stand by our policy. We do not routinely offer free or trial sized samples for testing purpo...

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Thank you for your inquiry. Yes, the antibody can be used for smoothe muscle.

Thanks you for your message. The deadline for placing orders to be dispatched before the holidays is midday December 18th. Please do let me know how soon you need the products. Would it be all right at the beginning of January? Or is it too late? ...

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This antibody will react with muscle and heart tissue, however it will not react with alpha-cardiac myosin. Furthermore this antibody has only been tested on human tissue and we suspect that it will not cross-react with mouse and/or rat although this h...

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All the information we have for antibodies to myosin, (ab7784, ab7829, ab7786, ab7785,) is in the datasheets

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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