Overview

  • Product nameAnti-Myosin antibody [C5C.S2]
    See all Myosin primary antibodies
  • Description
    Mouse monoclonal [C5C.S2] to Myosin
  • SpecificityThis antibody recognizes most forms of myosin II, including smooth muscle myosin, skeletal muscle myosin and myosin from non muscle cells (platelets and fibroblasts).
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    This antibody was raised against smooth muscle myosin.

  • EpitopeThe epitope is the S2 region of myosin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None.
    Constituents: Tissue culture supernatant.
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberC5C.S2
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB 1/5000. Predicted molecular weight: 205 kDa.

Target

References for Anti-Myosin antibody [C5C.S2] (ab24648)

This product has been referenced in:
  • Peacock JG  et al. The Abl-related gene tyrosine kinase acts through p190RhoGAP to inhibit actomyosin contractility and regulate focal adhesion dynamics upon adhesion to fibronectin. Mol Biol Cell 18:3860-72 (2007). ICC/IF ; Mouse . Read more (PubMed: 17652459) »
  • Ito M  et al. Effect of monoclonal antibodies on the properties of smooth muscle myosin. Biochemistry 28:5567-72 (1989). Read more (PubMed: 2528373) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

The tissue culture supernatant does contain all other proteins and IgGs as well, which is why it is hard to determine the concentration of specific IgG in these solutions.

I hope this information is he...

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Thank you for contacting us. I am sorry to hear your customer has been experiencing problems with one of our antibodies.  This antibody has been tested with a standard IP protocol and to our knowledge, customised protocols are not required for ...

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Thank you for contacting Abcam technical services regarding ab65867 - Anti-Mouse IgM.   I can confirm that this product has been tested for affinity purification only and we haven't tested it for immunoprecipitation specifically. As the principle i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"