Overview

  • Product nameAnti-Myosin antibody [MAC 147]
    See all Myosin primary antibodies
  • Description
    Rat monoclonal [MAC 147] to Myosin
  • SpecificityReacts with subfagment 2 of Lethocerus and Drosophila myosin
  • Tested applicationsSuitable for: ICC/IF, Electron Microscopy, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster, Lethocerus indicus (Waterbug)
  • Immunogen

    Flight muscle myosin purified from Lethocerus indicus (Waterbug)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberMAC 147
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51098 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 12 kDa.

Target

  • FunctionMuscle contraction.
  • Sequence similaritiesContains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localizationCytoplasm > myofibril. Thick filaments of the myofibrils.
  • Information by UniProt
  • Alternative names
    • adult 1 antibody
    • MYH1 antibody
    • MYH1_HUMAN antibody
    • MYH2 antibody
    • MYH3 antibody
    • MYH4 antibody
    • MYH6 antibody
    • MyHC-2x antibody
    • MyHC-IIx/d antibody
    • MYL1 antibody
    • MYL2 antibody
    • MYL3 antibody
    • MYL5 antibody
    • MYL6 antibody
    • Myosin light chain 2 regulatory cardiac slow antibody
    • Myosin 1 antibody
    • Myosin heavy chain 1 antibody
    • Myosin heavy chain 1 skeletal muscle adult antibody
    • Myosin heavy chain 2 skeletal muscle adult antibody
    • Myosin heavy chain 2x antibody
    • Myosin heavy chain 3 skeletal muscle adult antibody
    • Myosin heavy chain 4 skeletal muscle adult antibody
    • Myosin heavy chain 6 skeletal muscle adult antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain IIx/d antibody
    • Myosin heavy chain skeletal muscle adult 1 antibody
    • Myosin II antibody
    • Myosin light chain 1 alkali skeletal fast antibody
    • Myosin light chain 3 alkali ventricular skeletal slow antibody
    • Myosin light chain 4 alkali atrial embryonic antibody
    • Myosin light chain 5 regulatory antibody
    • Myosin light chain 6 alkali smooth muscle and non muscle antibody
    • Myosin-1 antibody
    • Myosin1 antibody
    • skeletal muscle antibody
    see all

Anti-Myosin antibody [MAC 147] images

  • ab51098 staining Myosin in Drosophila Melanogaster body wall muscle cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed prior to blocking in 1% BSA for 10 minutes at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 16 hours at 4°C. The secondary antibody was an undiluted Alexa Fluor® 647-conjugated Donkey anti-Rat polyclonal.

    See Abreview

References for Anti-Myosin antibody [MAC 147] (ab51098)

This product has been referenced in:
  • Molnár I  et al. DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila. PLoS Genet 10:e1004166 (2014). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 24586196) »
  • Hsu CY & Chan YP Identification and Localization of Proteins Associated with Biomineralization in the Iron Deposition Vesicles of Honeybees (Apis mellifera). PLoS One 6:e19088 (2011). WB ; Other . Read more (PubMed: 21541330) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Crab Cell (Mechanoreceptor neuron)
Specification Mechanoreceptor neuron
Permeabilization Yes - Tween
Fixative Formaldehyde
Username

Prof. Martin Mendelson

Verified customer

Submitted Jan 31 2014

Application Western blot
Sample Termite Tissue lysate - whole (PBS extracted ground termite)
Loading amount 10 µg
Specification PBS extracted ground termite
Treatment methoprene
Gel Running Conditions Reduced Denaturing (4-12% SDS-PAGE, DTT)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Chris Mattison

Verified customer

Submitted Sep 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (Body Wall Muslce)
Specification Body Wall Muslce
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Julie Ni

Verified customer

Submitted Nov 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"