Recombinant
RabMAb

Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721)

Overview

  • Product name
    Anti-Myosin Light Chain 2 antibody [EPR3741]
    See all Myosin Light Chain 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3741] to Myosin Light Chain 2
  • Tested applications
    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Myosin Light Chain 2 aa 150-250.

  • Positive control
    • WB: Fetal heart, fetal skeletal muscle, Human heart, mouse heart and rat heart lysates IHC-P: Human heart muscle
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 23365102
WB 1/5000. Predicted molecular weight: 19 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

The use of an HRP/AP polymerized secondary antibody is recommended.

  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Involvement in disease
      Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
      Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
    • Sequence similarities
      Contains 3 EF-hand domains.
    • Post-translational
      modifications
      N-terminus is methylated by METTL11A/NTM1.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiac myosin light chain-2 antibody
      • Cardiac ventricular myosin light chain 2 antibody
      • CMH10 antibody
      • MLC 2v antibody
      • MLC-2 antibody
      • MLC-2v antibody
      • MLC2 antibody
      • MLRV_HUMAN antibody
      • MYL 2 antibody
      • MYL2 antibody
      • Myosin light chain 2 regulatory cardiac slow antibody
      • Myosin light polypeptide 2 regulatory cardiac slow antibody
      • Myosin regulatory light chain 2 antibody
      • Myosin regulatory light chain 2 ventricular/cardiac muscle isoform antibody
      • Regulatory light chain of myosin antibody
      • RLC of myosin antibody
      • Slow cardiac myosin regulatory light chain 2 antibody
      • ventricular/cardiac muscle isoform antibody
      see all

    Images

    • All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

      Lane 1 : Fetal heart lysate
      Lane 2 : Fetal skeletal muscle lysate
      Lane 3 : Human heart lysate
      Lane 4 : Mouse heart lysate
      Lane 5 : Rat heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 19 kDa
    • ab92721, at a 1/100 dilution, staining Myosin Light Chain 2 in paraffin embedded Human heart muscle by Immunohistochemistry.
    • All lanes : Anti-Myosin Light Chain 2 antibody [EPR3741] (ab92721) at 1/10000 dilution

      Lane 1 : Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.1 µg
      Lane 2 : Recombinant Human Myosin Light Chain 2 protein (ab79185) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 4 minutes
    • ab92721 showing positive staining in Heart muscle tissue.

    • ab92721 showing positive staining in Skeletal muscle tissue.

    • ab92721 showing negative staining in Normal tonsil tissue.

    • ab92721 showing negative staining in Normal brain tissue.

    • ab92721 showing negative staining in Normal liver tissue.

    • ab92721 showing negative staining in Normal kidney tissue.

    • ab92721 showing negative staining in Normal breast tissue.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    • Xia K  et al. Effect of QSKL on MAPK and RhoA Pathways in a Rat Model of Heart Failure. Evid Based Complement Alternat Med 2017:3903898 (2017). WB ; Rat . Read more (PubMed: 28484504) »
    • Kudová J  et al. HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells. PLoS One 11:e0158358 (2016). Read more (PubMed: 27355368) »

    See all 11 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Oct 27 2015

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Ileon)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    Ileon
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 30 2015

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Rat Tissue lysate - whole (Skeletal muscle)
    Specification
    Skeletal muscle
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 22 2013

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Mouse Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 22 2013

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Human Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 22 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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