Anti-Myosin light chain 3 antibody [MLM508] (ab678)

Overview

  • Product nameAnti-Myosin light chain 3 antibody [MLM508]
    See all Myosin light chain 3 primary antibodies
  • Description
    Mouse monoclonal [MLM508] to Myosin light chain 3
  • SpecificityThis antibody reacts with ventricle myosin light chain I. Reactivity* Human ventricle myosin light chain I - 100% Human ventricle myosin light chain II - < 10% Human atrial myosin light chain I - < 1% Human atrial myosin light chain II - < 5% Human skeletal muscle myosin light chain - < 20% Human smooth muscle myosin light chain - < 0.1% * Assay was performed in combination with MLM544 and MLM508
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Cow, Human, Pig
  • Immunogen

    Human ventricle myosin light chain I

Properties

Applications

Our Abpromise guarantee covers the use of ab678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRegulatory light chain of myosin. Does not bind calcium.
  • Involvement in diseaseDefects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive.
    Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
  • Sequence similaritiesContains 3 EF-hand domains.
  • Post-translational
    modifications
    The N-terminus is blocked.
    N-terminus is methylated by METTL11A/NTM1.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cardiac myosin light chain 1 antibody
    • CMH8 antibody
    • CMLC1 antibody
    • ELC of myosin antibody
    • Essential light chain of myosin antibody
    • MLC1SB antibody
    • MLC1V antibody
    • MYL3 antibody
    • MYL3_HUMAN antibody
    • Myosin light chain 1 antibody
    • Myosin light chain 1 slow twitch muscle B/ventricular isoform antibody
    • Myosin light chain 1 slow, B antibody
    • Myosin light chain 3 antibody
    • Myosin, light chain 1, ventricular antibody
    • myosin, light chain 3, alkali; ventricular, skeletal, slow antibody
    • myosin, light polypeptide 3, alkali antibody
    • myosin, light polypeptide 3, alkali; ventricular, skeletal, slow antibody
    • OTTHUMP00000165922 antibody
    • Slow skeletal ventricular myosin alkali light chain 3 antibody
    • slow-twitch muscle B/ventricular isoform antibody
    • Ventricular/slow twitch myosin alkali light chain antibody
    • VLC1 antibody
    see all

Anti-Myosin light chain 3 antibody [MLM508] images

  • Procedure:
    Coat tube with MLM544
    Wash 2 times
    Block with BSA-PBS
    Add sample or standard (porcine cardiac myosin light chain I)
    Add 125I-labeled MLM508
    Incubate for 18 (12-24) h at 20 (10-20) °C
    Wash 2 times
    Measure radioactivity.

  • IHC image of ab678 staining in human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab678, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Myosin light chain 3 antibody [MLM508] (ab678)

This product has been referenced in:
  • Katoh H  et al. Development of an immunoradiometric assay kit for ventricular myosin light chain I with monoclonal antibodies. Clin Chem 38:170-1 (1992). Read more (PubMed: 1733600) »

See 1 Publication for this product

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Thank you for your enquiry. This antibody can react to the rat protein (myosin light chain I) almost equally to human and porcine protein in ELISA. If you have any additional questions, please contact us again.

Thank you for your email. For ab676, the concentration of cardiac MLI is 1 microgram/ml in PBS containing 1% BSA, 0.1% proclin. Purified material (> 90% purity) was diluted with the above buffer. For ab678, is has been tested in ELISA and does cross-re...

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Thank you for your enquiry and your interest in our products. I suggest that you conduct a search through the Abcam Website. I also recommend that you should click on the links “Product Reviews” and “Technical Enquiries” both accessible via the gree...

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