SpecificityThis antibody reacts with ventricle myosin light chain I.
Human ventricle myosin light chain I - 100%
Human ventricle myosin light chain II - < 10%
Human atrial myosin light chain I - < 1%
Human atrial myosin light chain II - < 5%
Human skeletal muscle myosin light chain - < 20%
Human smooth muscle myosin light chain - < 0.1%
Assay was performed in combination with MLM544 and MLM508
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
FunctionRegulatory light chain of myosin. Does not bind calcium.
Involvement in diseaseDefects in MYL3 are the cause of cardiomyopathy familial hypertrophic type 8 (CMH8) [MIM:608751]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. CMH8 inheritance can be autosomal dominant or recessive. Defects in MYL3 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 1 (MVC1) [MIM:608751]. MVC1 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.
Sequence similaritiesContains 3 EF-hand domains.
Post-translational modificationsThe N-terminus is blocked. N-terminus is methylated by METTL11A/NTM1.
Coat tube with ab677 Wash 2 times Block with BSA-PBS Add sample or standard (porcine cardiac myosin light chain I - ab676) Add 125I-labeled MLM508 Incubate for 18 (12-24) h at 20 (10-20) °C Wash 2 times Measure radioactivity.
References for Anti-Myosin light chain 3 antibody [MLM544] (ab677)
This product has been referenced in:
Katoh H et al. Development of an immunoradiometric assay kit for ventricular myosin light chain I with monoclonal antibodies. Clin Chem38:170-1 (1992).
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