Overview

  • Product nameAnti-Myosin light chain kinase antibody
    See all Myosin light chain kinase primary antibodies
  • Description
    Mouse monoclonal to Myosin light chain kinase
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: CTQCLQHPWL MKDTKNMEAK KLSKDRMKKY MARRKWQKTG NAVRAIGRLS SMAMISGLSG RKSSTGSPTS PLNAEKLESE EDVSQAFLEA VAEEKPHVKP , corresponding to amino acids 1710-1809 of Human Myosin light chain kinase

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCalcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
    • Tissue specificitySmooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 fibronectin type-III domain.
      Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      MLCK is probably down-regulated by phosphorylation.
      The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
    • Information by UniProt
    • Database links
    • Alternative names
      • deglutamylated form antibody
      • DKFZp686I10125 antibody
      • EC 2.7.11.18 antibody
      • FLJ12216 antibody
      • Kinase related protein antibody
      • Kinase-related protein antibody
      • KRP antibody
      • MLCK antibody
      • MLCK1 antibody
      • MLCK108 antibody
      • MLCK210 antibody
      • MSTP083 antibody
      • MYLK antibody
      • MYLK_HUMAN antibody
      • MYLK1 antibody
      • Myosin light chain kinase antibody
      • Myosin light polypeptide kinase antibody
      • OTTHUMP00000180642 antibody
      • OTTHUMP00000180643 antibody
      • smMLCK antibody
      • smooth muscle antibody
      • Smooth muscle myosin light chain kinase antibody
      • Telokin antibody
      see all

    Anti-Myosin light chain kinase antibody images

    • Western blot against tagged recombinant protein immunogen using ab55475 Myosin light chain kinase antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-Myosin light chain kinase antibody (ab55475)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Bladder cancer cells)
    Loading amount 30 µg
    Specification Bladder cancer cells
    Gel Running Conditions Non-reduced Denaturing (8)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Dr. santosh chauhan

    Verified customer

    Submitted Aug 03 2011

    Application Western blot
    Sample Human Cell lysate - whole cell (Raji)
    Loading amount 60 µg
    Specification Raji
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 09 2009

    Application Western blot
    Sample Human Cell lysate - whole cell (myometrium)
    Loading amount 10 µg
    Specification myometrium
    Gel Running Conditions Reduced Denaturing (12% Bis-Tris, Precast gel)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Dr. Jonathan Paul

    Verified customer

    Submitted Jun 03 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"