Anti-Myosin light chain kinase antibody [EP1458Y] (ab76092)

Overview

  • Product nameAnti-Myosin light chain kinase antibody [EP1458Y]
    See all Myosin light chain kinase primary antibodies
  • Description
    Rabbit monoclonal [EP1458Y] to Myosin light chain kinase
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C-terminal of human Myosin light chain kinase

  • Positive control
    • WB: Human brain lysate IHC-P: Human smooth muscle tissue IF: SH-SY5Y cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEP1458Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Predicted molecular weight: 211 kDa.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.

  • Application notesIs unsuitable for IP.
  • Target

    • FunctionCalcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
    • Tissue specificitySmooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
      Contains 1 fibronectin type-III domain.
      Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      MLCK is probably down-regulated by phosphorylation.
      The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
    • Information by UniProt
    • Database links
    • Alternative names
      • deglutamylated form antibody
      • DKFZp686I10125 antibody
      • EC 2.7.11.18 antibody
      • FLJ12216 antibody
      • Kinase related protein antibody
      • Kinase-related protein antibody
      • KRP antibody
      • MLCK antibody
      • MLCK1 antibody
      • MLCK108 antibody
      • MLCK210 antibody
      • MSTP083 antibody
      • MYLK antibody
      • MYLK_HUMAN antibody
      • MYLK1 antibody
      • Myosin light chain kinase antibody
      • Myosin light polypeptide kinase antibody
      • OTTHUMP00000180642 antibody
      • OTTHUMP00000180643 antibody
      • smMLCK antibody
      • smooth muscle antibody
      • Smooth muscle myosin light chain kinase antibody
      • Telokin antibody
      see all

    Anti-Myosin light chain kinase antibody [EP1458Y] images

    • ab76092 staining Myosin light chain kinase in mouse oocyte cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 overnight at 4°C and blocked with 3% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/50) for 12 hours at 4°C. A Cy5®-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • Immunohistochemical analysis of paraffin embedded human smooth muscle, using ab76092, at 1/100 dilution.

    References for Anti-Myosin light chain kinase antibody [EP1458Y] (ab76092)

    This product has been referenced in:
    • Liang QX  et al. Deletion of Mylk1 in oocytes causes delayed morula-to-blastocyst transition and reduced fertility without affecting folliculogenesis and oocyte maturation in mice. Biol Reprod 92:97 (2015). ICC/IF ; Mouse . Read more (PubMed: 25761595) »
    • Liu FF  et al. Characteristics of diprophylline-induced bidirectional modulation on rat jejunal contractility. Korean J Physiol Pharmacol 18:47-53 (2014). WB ; Rat . Read more (PubMed: 24634596) »

    See all 8 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Embryonic Heart)
    Permeabilization Yes - Tween-20
    Specification Embryonic Heart
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    Submitted Oct 31 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Heart)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 30 µg
    Specification Heart
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Oct 20 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (colon from Nonalcoholic Steatohepatitis mouse)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.5% typsin
    Specification colon from Nonalcoholic Steatohepatitis mouse
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative Formaldehyde
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    Submitted Dec 22 2015

    Application Western blot
    Sample Mouse Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount 28 µg
    Specification Intestinal tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Oct 16 2015

    Application Western blot
    Sample Rat Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions Reduced Denaturing (4-12% BIS TRIS)
    Loading amount 20 µg
    Specification Intestinal tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Oct 16 2015

    Application Western blot
    Sample Human Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount 28 µg
    Specification Intestinal tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Sep 21 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (Oocyte)
    Permeabilization Yes - 0.1%triton 4? overnight
    Specification Oocyte
    Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative Paraformaldehyde
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    Submitted Jun 12 2015

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
    Sample Human Cell (Human arterial myocytes)
    Specification Human arterial myocytes
    Permeabilization Yes - 0.3% Triton X-100
    Fixative Paraformaldehyde
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    Submitted Apr 08 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (8% gel was used)
    Sample Rat Tissue lysate - whole (jejunal segment)
    Specification jejunal segment
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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    Dapeng Chen

    Verified customer

    Submitted Sep 13 2013



    The immunogen of ab76092 is specific for MLCK1 and shows no similarity with MLCK2. It was taken from a region that is contained within all isoforms of MLCK1, so it will recognize all of these isoforms.

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