The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 20 kDa.
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
Smooth muscle tissues and in some, but not all, nonmuscle cells.
Contains 3 EF-hand domains.
Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.
Immunofluorescent analysis of paraformaldehyde-fixed A7r5 cells labeling Myosin light chain with ab157747 at 1/100 dilution (left). Cells were dual stained with mouse anti-Myosin light chain antibody (middle panel) and the overlay of staining patterns is shown to the right. Goat anti-mouse Dylight 488 and goat anti-rabbit Dylight 594 were used for detection of primary antibodies.
Western blot - Anti-Myosin light chain (phospho S1) antibody (ab157747)
All lanes : Anti-Myosin light chain (phospho S1) antibody (ab157747)
Lane 1 : C2C12 cell lysate, untreated Lane 2 : C2C12 cell lysate treated with Lambda phosphatase