Anti-Myosin Phosphatase antibody (ab111237)

Overview

  • Product nameAnti-Myosin Phosphatase antibody
    See all Myosin Phosphatase primary antibodies
  • Description
    Goat polyclonal to Myosin Phosphatase
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, correspondin to residues in Human Myosin Phosphatase.

  • Positive control
    • Human skeletal muscle tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab111237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRegulates myosin phosphatase activity.
  • Tissue specificityExpressed in striated muscles, specifically in type 2a fibers (at protein level).
  • Sequence similaritiesContains 6 ANK repeats.
  • Developmental stageInduced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • DomainHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • Post-translational
    modifications
    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • Cellular localizationCytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Anti-Myosin Phosphatase antibody images

  • ab111237, at 3.75 µg/ml, staining Myosin Phosphatase in formalin fixed, paraffin embedded Human skeletal muscle tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase streptavidin and chromogen.

References for Anti-Myosin Phosphatase antibody (ab111237)

ab111237 has not yet been referenced specifically in any publications.

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