Overview

  • Product nameAnti-Myosin Phosphatase antibody
    See all Myosin Phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Myosin Phosphatase
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Myosin Phosphatase around the phosphorylation site of threonine 853 (R-S-TP-G-V).

  • Positive control
    • Human brain tissue and NIH/3T3 cell extracts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).

Target

  • FunctionRegulates myosin phosphatase activity.
  • Tissue specificityExpressed in striated muscles, specifically in type 2a fibers (at protein level).
  • Sequence similaritiesContains 6 ANK repeats.
  • Developmental stageInduced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • DomainHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • Post-translational
    modifications
    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • Cellular localizationCytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Anti-Myosin Phosphatase antibody images

  • All lanes : Anti-Myosin Phosphatase antibody (ab59235) at 1/500 dilution

    Lane 1 : NIH3T3 cell extract
    Lane 2 : NIH3T3 cell extract with immunising peptide


    Predicted band size : 115 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • ab59235 at 1/50 dilution staining Myosin Phosphatase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-Myosin Phosphatase antibody (ab59235)

This product has been referenced in:
  • Fu K  et al. Tra2ß Protein Is Required for Tissue-specific Splicing of a Smooth Muscle Myosin Phosphatase Targeting Subunit Alternative Exon. J Biol Chem 287:16575-85 (2012). WB ; Mouse . Read more (PubMed: 22437831) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"