The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
Use at 2-5 µg/mg of lysate.
FunctionRegulates myosin phosphatase activity.
Tissue specificityExpressed in striated muscles, specifically in type 2a fibers (at protein level).
Sequence similaritiesContains 6 ANK repeats.
Developmental stageInduced by 2-fold during pregnancy, including in abdominus rectus muscle.
DomainHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
Post-translational modificationsPhosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
Cellular localizationCytoplasm. Along actomyosin filaments and stress fibers.
1mg whole lysate from HeLa cells was immunoprecipitated using ab70809 at 3ug/mg of lysate (lane 1) or a control rabbit Ig (lane 2). For the subsequent western blot, 20% of the immunoprecipitate was loaded per lane, and probed with ab70809 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.
References for Anti-Myosin Phosphatase antibody (ab70809)
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