Anti-Myosin Phosphatase antibody [Y110] (ab32393)


  • Product name
    Anti-Myosin Phosphatase antibody [Y110]
    See all Myosin Phosphatase primary antibodies
  • Description
    Rabbit monoclonal [Y110] to Myosin Phosphatase
  • Specificity
    Ab32393 recognises Myosin Phosphatase. It can detect all four splice isoforms of Myosin Phosphatase.
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in the first ANK repeat of human Myosin Phosphatase.

  • Positive control
    • 293 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab32393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC or IP.
  • Target

    • Function
      Regulates myosin phosphatase activity.
    • Tissue specificity
      Expressed in striated muscles, specifically in type 2a fibers (at protein level).
    • Sequence similarities
      Contains 6 ANK repeats.
    • Developmental stage
      Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
    • Domain
      Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
    • Post-translational
      Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
    • Cellular localization
      Cytoplasm. Along actomyosin filaments and stress fibers.
    • Information by UniProt
    • Database links
    • Alternative names
      • M130 antibody
      • MBS antibody
      • Myosin binding subunit antibody
      • Myosin phosphatase target subunit 1 antibody
      • Myosin phosphatase targeting subunit 1 antibody
      • Myosin phosphatase-targeting subunit 1 antibody
      • MYPT1 antibody
      • MYPT1_HUMAN antibody
      • PPP1R12A antibody
      • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
      • Protein phosphatase 1 regulatory subunit 12A antibody
      • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
      • Protein phosphatase myosin binding subunit antibody
      • Protein phosphatase myosin-binding subunit antibody
      see all

    Anti-Myosin Phosphatase antibody [Y110] images

    References for Anti-Myosin Phosphatase antibody [Y110] (ab32393)

    This product has been referenced in:
    • Petrakova OS  et al. Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells. Springerplus 3:183 (2014). ICC/IF ; Mouse . Read more (PubMed: 24790827) »
    • Kim JH & Adelstein RS LPA(1) -induced migration requires nonmuscle myosin II light chain phosphorylation in breast cancer cells. J Cell Physiol 226:2881-93 (2011). Read more (PubMed: 21302283) »

    See all 2 Publications for this product

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