The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 125 kDa (predicted molecular weight: 115 kDa).
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt,IHC or IP.
FunctionRegulates myosin phosphatase activity.
Tissue specificityExpressed in striated muscles, specifically in type 2a fibers (at protein level).
Sequence similaritiesContains 6 ANK repeats.
Developmental stageInduced by 2-fold during pregnancy, including in abdominus rectus muscle.
DomainHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
Post-translational modificationsPhosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
Cellular localizationCytoplasm. Along actomyosin filaments and stress fibers.