Anti-Myosin Phosphatase (phospho T696) antibody (ab59202)


  • Product nameAnti-Myosin Phosphatase (phospho T696) antibody
    See all Myosin Phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Myosin Phosphatase (phospho T696)
  • SpecificityDetects endogenous levels of Myosin Phosphatase only when phosphorylated at threoine 696.
  • Tested applicationsSuitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 696 (RSTPQG)

  • Positive control
    • Human breast carcinoma tissue


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab59202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.


  • FunctionRegulates myosin phosphatase activity.
  • Tissue specificityExpressed in striated muscles, specifically in type 2a fibers (at protein level).
  • Sequence similaritiesContains 6 ANK repeats.
  • Developmental stageInduced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • DomainHeterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • Post-translational
    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • Cellular localizationCytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Anti-Myosin Phosphatase (phospho T696) antibody images

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59202 at 1/50 dilution. Samples were untreated (left) or treated with immunizing peptide (right).

References for Anti-Myosin Phosphatase (phospho T696) antibody (ab59202)

ab59202 has not yet been referenced specifically in any publications.

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Thank you for your inquiry.

We don't have a polymer kit besides our mouse on mouse polymer kit, ab127055.

We have ABC kits and EXPOSE kits.


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Thank you for your reply.

This is just to go along with the polymer kit that was used. If you are using polymer detection, simply follow the manufacturers instructions. You can omit this step if not using this type of amplification.

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Thank you for contacting Abcam regarding ab59202.

The IHC protocol is as follows:


1. Incubate slide at 60℃ for 60 minutes.

2. Deparaffinize in Xylene for 10 minutes and repeat on...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 120 µg
Specification Hela
Treatment 10ng/ml IL6 for 30 minutes
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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Submitted Sep 02 2008