Anti-Myosin Phosphatase (phospho T696) antibody (ab59202)

Overview

  • Product name
    Anti-Myosin Phosphatase (phospho T696) antibody
    See all Myosin Phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Myosin Phosphatase (phospho T696)
  • Specificity
    Detects endogenous levels of Myosin Phosphatase only when phosphorylated at threoine 696.
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 696 (RSTPQG)

  • Positive control
    • Human breast carcinoma tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Function
    Regulates myosin phosphatase activity.
  • Tissue specificity
    Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • Sequence similarities
    Contains 6 ANK repeats.
  • Developmental stage
    Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • Domain
    Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • Post-translational
    modifications
    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • Cellular localization
    Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Anti-Myosin Phosphatase (phospho T696) antibody images

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab59202 at 1/50 dilution. Samples were untreated (left) or treated with immunizing peptide (right).

References for Anti-Myosin Phosphatase (phospho T696) antibody (ab59202)

This product has been referenced in:
  • Sastre E  et al. Decompensated liver cirrhosis and neural regulation of mesenteric vascular tone in rats: role of sympathetic, nitrergic and sensory innervations. Sci Rep 6:31076 (2016). WB ; Rat . Read more (PubMed: 27484028) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

We don't have a polymer kit besides our mouse on mouse polymer kit, ab127055.

http://www.abcam.com/Mouse-on-Mouse-MOM-Polymer-IHC-Kit-ab127055.html

We have ABC kits and EXPOSE kits.

http:/...

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Thank you for your reply.


This is just to go along with the polymer kit that was used. If you are using polymer detection, simply follow the manufacturers instructions. You can omit this step if not using this type of amplification.
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Thank you for contacting Abcam regarding ab59202.



The IHC protocol is as follows:



Deparaffinization

1. Incubate slide at 60℃ for 60 minutes.

2. Deparaffinize in Xylene for 10 minutes and repeat on...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela)
Loading amount
120 µg
Specification
Hela
Treatment
10ng/ml IL6 for 30 minutes
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Verified customer

Submitted Sep 02 2008

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