Anti-Myosin Phosphatase (phospho T853) antibody (ab59203)

Overview

  • Product name
    Anti-Myosin Phosphatase (phospho T853) antibody
    See all Myosin Phosphatase primary antibodies
  • Description
    Rabbit polyclonal to Myosin Phosphatase (phospho T853)
  • Specificity
    Detects human Myosin Phosphatase only when phosphorylated at threonine 853 (threonine 852 for mouse and threonine 855 for rat)
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 853 (RSTPGV)

  • Positive control
    • Human colon carcinoma. NIH/3T3 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 115 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

Target

  • Function
    Regulates myosin phosphatase activity.
  • Tissue specificity
    Expressed in striated muscles, specifically in type 2a fibers (at protein level).
  • Sequence similarities
    Contains 6 ANK repeats.
  • Developmental stage
    Induced by 2-fold during pregnancy, including in abdominus rectus muscle.
  • Domain
    Heterotetramerization is mediated by the interaction between a coiled-coil of PRKG1 and the leucine/isoleucine zipper of PPP1R12A/MBS, the myosin-binding subunit of the myosin phosphatase.
  • Post-translational
    modifications
    Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
    In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1.
  • Cellular localization
    Cytoplasm. Along actomyosin filaments and stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • M130 antibody
    • MBS antibody
    • Myosin binding subunit antibody
    • Myosin phosphatase target subunit 1 antibody
    • Myosin phosphatase targeting subunit 1 antibody
    • Myosin phosphatase-targeting subunit 1 antibody
    • MYPT1 antibody
    • MYPT1_HUMAN antibody
    • PPP1R12A antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 12A antibody
    • Protein phosphatase 1 regulatory subunit 12A antibody
    • Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody
    • Protein phosphatase myosin binding subunit antibody
    • Protein phosphatase myosin-binding subunit antibody
    see all

Images

  • All lanes : Anti-Myosin Phosphatase (phospho T853) antibody (ab59203) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extract
    Lane 2 : NIH/3T3 cell extract with immunizing phosphopeptide


    Predicted band size : 115 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59203 at 1/50 dilution. Samples were treated -/+ phosphopeptide.

References

This product has been referenced in:
  • Wang XQ  et al. Resveratrol enhances vascular reactivity in mice following lipopolysaccharide challenge via the RhoA-ROCK-MLCP pathway. Exp Ther Med 14:308-316 (2017). Read more (PubMed: 28672931) »
  • Cheng CI  et al. The prognostic values of leukocyte rho kinase activity in acute ischemic stroke. Biomed Res Int 2014:214587 (2014). WB ; Human . Read more (PubMed: 24716192) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for submitting an Abreview of ab59203. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up o...

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Application
Western blot
Sample
Mouse Tissue lysate - whole (Left ventricle)
Loading amount
20 µg
Specification
Left ventricle
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Aug 29 2012

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