Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)

Overview

  • Product nameAnti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335]
    See all Myosin, smooth muscle heavy chain 1 and 2 primary antibodies
  • Description
    Rabbit monoclonal [EPR5335] to Myosin, smooth muscle heavy chain 1 and 2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Myosin, smooth muscle heavy chain 1 and 2.

  • Positive control
    • WB: Human fetal artery, bladder and mouse spleen lysates IHC: Human colon and kidney tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 1.85 x 10 -10 M Learn more about KD
  • Storage bufferpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR5335
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 250 kDa (predicted molecular weight: 227 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Target

  • FunctionMuscle contraction.
  • Tissue specificitySmooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • Involvement in diseaseNote=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • Sequence similaritiesContains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localizationMelanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • KIAA0866 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SM1 antibody
    • SM2 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 antibody
    see all

Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] images

  • Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/20000 dilution (purified) + mouse spleen at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size : 227 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/50000 dilution (purified) + human fetal artery lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 227 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human smooth muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Unpurified ab124679, at 1/100 dilution, staining Myosin, smooth muscle heavy chain 1 and 2 in paraffin-embedded Human colon tissue by Immunohistochemistry.

  • Unpurified ab124679, at 1/100 dilution, staining Myosin, smooth muscle heavy chain 1 and 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

  • All lanes : Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679) at 1/10000 dilution (unpurified)

    Lane 1 : Human fetal artery lysate
    Lane 2 : Human bladder lysate
    Lane 3 : Mouse spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 227 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [EPR5335] (ab124679)

This product has been referenced in:
  • Wang D  et al. Local MicroRNA Modulation Using a Novel Anti-miR-21-Eluting Stent Effectively Prevents Experimental In-Stent Restenosis. Arterioscler Thromb Vasc Biol 35:1945-53 (2015). IHC . Read more (PubMed: 26183619) »
  • Castellanos-Rivera RM  et al. Recombination Signal Binding Protein for Ig-?J Region Regulates Juxtaglomerular Cell Phenotype by Activating the Myo-Endocrine Program and Suppressing Ectopic Gene Expression. J Am Soc Nephrol N/A:N/A (2014). Read more (PubMed: 24904090) »

See all 2 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"