Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SMMS-1] (ab106919)

Overview

  • Product name
    Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SMMS-1]
    See all Myosin, smooth muscle heavy chain 1 and 2 primary antibodies
  • Description
    Mouse monoclonal [SMMS-1] to Myosin, smooth muscle heavy chain 1 and 2
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Crude Human uterus extract

  • Positive control
    • Uterus or normal breast tissue. Some breast cancers, leiomyosarcoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab106919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • Function
    Muscle contraction.
  • Tissue specificity
    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • Involvement in disease
    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization
    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • KIAA0866 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SM1 antibody
    • SM2 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 antibody
    see all

References for Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SMMS-1] (ab106919)

This product has been referenced in:
  • Nicolas MM  et al. Pleomorphic and dedifferentiated leiomyosarcoma: clinicopathologic and immunohistochemical study of 41 cases. Hum Pathol 41:663-71 (2010). Read more (PubMed: 20004935) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human smooth muscle cells)
Specification
human smooth muscle cells
Fixative
Formaldehyde; Methanol; paraformaldehyde
Permeabilization
Yes - Triton
Blocking step
BSA/Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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