Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SPM201], prediluted (ab17811)

Overview

  • Product nameAnti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SPM201], prediluted
    See all Myosin, smooth muscle heavy chain 1 and 2 primary antibodies
  • Description
    Mouse monoclonal [SPM201] to Myosin, smooth muscle heavy chain 1 and 2, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Crude human uterus extract

  • Positive control
    • Crude human uterus extract
  • General notesTwo isoforms: 205kDa (MHC-1) and 200kDa (MHC-2)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
  • PurityWhole antiserum
  • ClonalityMonoclonal
  • Clone numberSPM201
  • IsotypeIgG
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: Working dilution: Ready-to-use for 30 min at RT
    Staining of formalin-fixed tissues REQUIRES digesting sections with trypsin at 1mg/ml PBS, 10 min at 37oC, followed by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMuscle contraction.
    • Tissue specificitySmooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
    • Involvement in diseaseNote=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
      Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
    • Sequence similaritiesContains 1 IQ domain.
      Contains 1 myosin head-like domain.
    • DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
      Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
    • Cellular localizationMelanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
    • Information by UniProt
    • Database links
    • Alternative names
      • AAT4 antibody
      • DKFZp686D10126 antibody
      • DKFZp686D19237 antibody
      • FAA4 antibody
      • FLJ35232 antibody
      • KIAA0866 antibody
      • MGC126726 antibody
      • MGC32963 antibody
      • MYH11 antibody
      • MYH11_HUMAN antibody
      • Myosin heavy chain 11 antibody
      • Myosin heavy chain 11 smooth muscle antibody
      • Myosin heavy chain antibody
      • Myosin heavy chain smooth muscle isoform antibody
      • Myosin heavy polypeptide 11 smooth muscle antibody
      • Myosin-11 antibody
      • SM1 antibody
      • SM2 antibody
      • SMHC antibody
      • SMMHC antibody
      • smooth muscle isoform antibody
      • Smooth muscle myosin heavy chain 11 antibody
      see all

    Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SPM201], prediluted images

    • Human uterus stained with anti-Myosin, (ab17811) 

    References for Anti-Myosin, smooth muscle heavy chain 1 and 2 antibody [SPM201], prediluted (ab17811)

    ab17811 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab17811.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"