Overview

  • Product name
  • Description
    Rabbit polyclonal to Myosin VIIa
  • Specificity
    Detects Myosin VIIa from mouse tissues as well as recombinant. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa from mouse testes preparations. This antibody detects recombinant mouse myosin VIIa overexpressed in Sf9 insect cell lysate.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC, IP, WB, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Mouse Myosin VIIa aa 16-31.
    Sequence:

    SGQEFDVPIGAVVKLC


    (Peptide available as ab4996)

  • Positive control
    • WB: mouse testes lysate IHC-P: mouse ear

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 3-5µg for 106 cells.
ICC 1/50.
IP Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 220 kDa.Can be blocked with Myosin VIIa peptide (ab4996).
ICC/IF Use at an assay dependent concentration. PubMed: 19429027
IHC-Fr Use at an assay dependent concentration. PubMed: 20461409

Target

  • Relevance
    Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
  • Cellular localization
    Cytoplasmic, cytoskeleton
  • Database links
  • Alternative names
    • Deafness autosomal dominant 11 antibody
    • Deafness autosomal recessive 2 antibody
    • DFNA11 antibody
    • DFNB 2 antibody
    • DFNB2 antibody
    • Myo7a antibody
    • Myosin 7a antibody
    • Myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)) antibody
    • Myosin VIIa antibody
    • Myosin, unconventional, family VII, member A antibody
    • MYOVIIA antibody
    • MYU7A antibody
    • NSRD 2 antibody
    • NSRD2 antibody
    • Unconventional myosin VIIa antibody
    • Ush 1B antibody
    • Ush1b antibody
    • Usher syndrome 1B antibody
    see all

Anti-Myosin VIIa antibody images

  • ab3481 staining primary cell culture of Pig retinal pigment epithelium by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C.  The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 4°C.  An Alexa Fluor® 148 conjugated goat anti-rabbit antibody, diluted 1/750, was used as the secondary.

    See Abreview

  • Anti-Myosin VIIa antibody (ab3481) at 5 µg/ml + Membrane enriched extracts of L6 at 30 µg with Skimmed milk at 5 %

    Secondary
    HRP conjugated goat anti-rabbit IgG (H+L) at 1/4000 dilution
  • (G) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin. (H) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with rabbit IgG as an isotype control and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.

  • (A) Histochemical staining of mouse P5 cochlear sensory epithelia inner hair cells (IHC) and outer hair cells (OHC) with Alexa Fluor 488® phalloidin.  (B) Immunohistochemical staining of mouse P5 cochlear sensory epithelia with ab3481 as the primary antibody (dilution 1/900) and an Alexa Fluor 594®-conjugated goat anti-rabbit antibody as the secondary antibody (dilution 1/2500). The scale bars represent 8 μm.

  • ab3481 at a 1/500 dilution staining Myosin VIIa in Mouse retinal pigment epithelium primary cells by Immunocytochemistry/ Immunofluorescence, incubated for 16 hours at 4°C in 1% goat serum, 0.1% Triton X-100, 1X PBS. Fixed in formalin. Permeabilized using 0.5% Triton X-100. Blocked with 5% serum for 20 minutes at 25°C. Secondary used at 1/500 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.

    See Abreview

  • Western blot detection of Myosin VIIa in mouse testes tissue extract using ab3481.

  • ab3481 staining Myosin VIIa (red histogram) on L6 cells by Flow Cytometry. Cells were fixed with 70% ethonal, permeabilized with Triton and blocked with 5% BSA for 30 minutes at room temperature. The sample was incubated with the primary antibody (3-5 ug/10^6 in 2.5% BSA) for 2 hours at room temperature. An Alexa fluor® 488-conjugated Goat anti-rabbit IgG (1/400) was used as the secondary antibody. Purple histogram represents unstained control, pink histogram represents rabbit isotype control and green histogram represents no primary antibody control

     

References for Anti-Myosin VIIa antibody (ab3481)

This product has been referenced in:
  • Fan F  et al. Exophilin-8 assembles secretory granules for exocytosis in the actin cortex via interaction with RIM-BP2 and myosin-VIIa. Elife 6:N/A (2017). Read more (PubMed: 28673385) »
  • Schmitz HM  et al. Kanamycin-furosemide ototoxicity in the mouse cochlea: a 3-dimensional analysis. Otolaryngol Head Neck Surg 150:666-72 (2014). Mouse . Read more (PubMed: 24415490) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (MDCK II)
Specification
MDCK II
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TX100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Retinal Pigment Epithelium primary cells)
Specification
Retinal Pigment Epithelium primary cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% TX100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 26 2009

Thank you for your enquiry. There is no stated expiration date for this antibody. There is one year expiration date from the date of purchase, however the Abpromise will be still effective for only 120 days after purchase. This antibody is unli...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary cell culture, retinal pigment epithelium)
Specification
Primary cell culture, retinal pigment epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TX100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Dec 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Hamster Cell (CHO, chinese hamster ovary cells)
Specification
CHO, chinese hamster ovary cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Dec 02 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (ear cross sections)
Specification
ear cross sections
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Mrs. Chantal Longo-Guess

Verified customer

Submitted Jun 10 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Primary cell culture, retinal pigment epithelium)
Specification
Primary cell culture, retinal pigment epithelium
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TX100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jun 05 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (Pig RPE cells)
Loading amount
25 µg
Specification
Pig RPE cells
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Jun 03 2008

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet and we are unaware if the sequence is also conserved in zebrafish.

Thank you for your enquiry. This antibody has not yet been tested for use with immunofluorescence. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift vo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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