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Rabbit monoclonal [EPR7497] to Myosin VIIa
Flow Cyt more details Unsuitable for:
IHC-P or IP
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
Rat and mouse testis lysates; Y79 and A97 cells
This product is a recombinant rabbit monoclonal antibody.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 254 kDa.
1/100 - 1/250.
1/100 - 1/500.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IHC-P or IP.
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. In retina, myosin VIIa may play a role in trafficking of ribbon-synaptic vesicle complexes and renewal of the outer photoreceptors disks. In inner ear, it may maintain the rigidity of stereocilia during the dynamic movements of the bundle.
Deafness autosomal dominant 11 antibody
Deafness autosomal recessive 2 antibody
Western blot - Anti-Myosin VIIa antibody [EPR7497] (ab150386)
All lanes : Anti-Myosin VIIa antibody [EPR7497] (ab150386) at 1/1000 dilution Lane 1 : Y79 cell lysate Lane 2 : rat testis cell lysate Lane 3 : mouse testis cell lysate Lysates/proteins at 10 µg per lane. Secondary HRP labelled goat-anti rabbit antibody at 1/2000 dilution Predicted band size : 254 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Myosin VIIa antibody [EPR7497] (ab150386)
Immunofluorescence analysis of A97 cells labeling Myosin VIIa with ab150386 at a 1/100 dilution.
Flow Cytometry - Anti-Myosin VIIa antibody [EPR7497] (ab150386)
Flow cytometric analysis of permeabilized Y79 cells labeling Myosin VIIa with ab150386 at a 1/100 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"